Detailed information of TA system TA167636

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1917493..1918327
• Replicon: chromosome
• Accession: NZ_CP058833
• Organism: Shigella flexneri strain STLEFF_27

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2I6F3V8
• Locus tag: HZT22_RS09375
• Protein ID: WP_054627366.1
• Coordinates: 1917950..1918327 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A4Q1HU91
• Locus tag: HZT22_RS09370
• Protein ID: WP_053287563.1
• Coordinates: 1917493..1917861 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT22_RS09350 (1915238)	1915238..1916056	+	819	WP_096200128.1	DUF932 domain-containing protein	-
HZT22_RS09355 (1916147)	1916147..1916632	+	486	WP_000214317.1	antirestriction protein	-
HZT22_RS09360 (1916647)	1916647..1917123	+	477	WP_001186747.1	RadC family protein	-
HZT22_RS09365 (1917192)	1917192..1917413	+	222	WP_000692312.1	DUF987 domain-containing protein	-
HZT22_RS09370 (1917493)	1917493..1917861	+	369	WP_053287563.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HZT22_RS09375 (1917950)	1917950..1918327	+	378	WP_054627366.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
HZT22_RS09380 (1918324)	1918324..1918473	+	150	Protein_1846	DUF5983 family protein	-
HZT22_RS09385 (1918549)	1918549..1918746	+	198	WP_023563519.1	DUF957 domain-containing protein	-
HZT22_RS09390 (1918831)	1918831..1919676	+	846	WP_063101552.1	DUF4942 domain-containing protein	-
HZT22_RS09400 (1920410)	1920410..1922662	+	2253	Protein_1849	alpha-amylase family glycosyl hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13928.97 Da
• Isoelectric Point: 8.5163

>T167636 WP_054627366.1 NZ_CP058833:1917950-1918327 [Shigella flexneri]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T167636 NZ_CP075257:36656-37126 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13663.36 Da
• Isoelectric Point: 6.4768

>AT167636 WP_053287563.1 NZ_CP058833:1917493-1917861 [Shigella flexneri]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNSRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT167636 NZ_CP075257:36216-36659 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A2I6F3V8

Antitoxin

• AlphaFold DB: A0A4Q1HU91