Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4180568..4181065 Replicon chromosome
Accession NZ_CP058831
Organism Shigella flexneri strain STLE4

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HZT21_RS20400 Protein ID WP_001173343.1
Coordinates 4180568..4180741 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HZT21_RS20405 Protein ID WP_000398619.1
Coordinates 4180769..4181065 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT21_RS20390 (4177238) 4177238..4178695 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HZT21_RS20395 (4178932) 4178932..4180449 + 1518 WP_001295074.1 lysine--tRNA ligase -
HZT21_RS20400 (4180568) 4180568..4180741 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HZT21_RS20405 (4180769) 4180769..4181065 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HZT21_RS20410 (4181292) 4181292..4181564 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
HZT21_RS20415 (4181576) 4181576..4181806 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
HZT21_RS20420 (4181987) 4181987..4183618 + 1632 WP_001216477.1 sensor histidine kinase -
HZT21_RS20425 (4183615) 4183615..4184334 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T167621 WP_001173343.1 NZ_CP058831:c4180741-4180568 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T167621 NZ_CP075192:3181689-3181865 [Ferribacterium limneticum]
TTGCGCAAGGTGCGCTGGAGCCGGGCCGGTTCGGGCAAGTCCGGCGGTGTGCGGGTGATTTATTTCACCCGCAACGCGGT
GGGTGAGATTGTGTTGCTGTTGCTGTACGCCAAATCCGAGCGGGACTCGATGACCGCCAAACAATTGCTGGAGATTAAAA
ATGCCCTCGACCTCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT167621 WP_000398619.1 NZ_CP058831:c4181065-4180769 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT167621 NZ_CP075192:3181849-3182160 [Ferribacterium limneticum]
ATGCCCTCGACCTCTAATCCAATGTCCGATGATGATCTGGCTGCCTTTGAGGCGGGCCGGGATTTGCACGCAGATTTGCT
CCAGTCCATTCGAGAAATGAAGGCAGGGCGCGGTGTTGTGGTGCTTTCGCCAGTAATTGCCGCGCGCAAGGCTTCGGGTT
TGTCCCAGTCACAGTTTGCCGAACTGATGGGGGTTTCGGTGCGCACCCTGCAGGAGTGGGAGCAAGGCCGCCGCCAGCCT
TCCGGCGCGGCCTTGACGCTGCTCAAGGTGGCAGAGCGCCACCCGGAGGTACTGCGGGAGCTGGCTGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References