Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3949771..3950347 Replicon chromosome
Accession NZ_CP058831
Organism Shigella flexneri strain STLE4

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HZT21_RS19225 Protein ID WP_001297643.1
Coordinates 3950060..3950347 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HZT21_RS19220 Protein ID WP_000063156.1
Coordinates 3949771..3950073 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT21_RS19205 (3946367) 3946367..3948517 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
HZT21_RS19210 (3948567) 3948567..3948770 + 204 WP_000467859.1 YbdD/YjiX family protein -
HZT21_RS19215 (3948781) 3948781..3949737 + 957 WP_001297640.1 GTPase -
HZT21_RS19220 (3949771) 3949771..3950073 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HZT21_RS19225 (3950060) 3950060..3950347 - 288 WP_001297643.1 BrnT family toxin Toxin
HZT21_RS19230 (3950546) 3950546..3951243 - 698 Protein_3776 IS1 family transposase -
HZT21_RS19235 (3951300) 3951300..3951395 + 96 Protein_3777 dienelactone hydrolase family protein -
HZT21_RS19240 (3951541) 3951541..3952773 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
HZT21_RS19245 (3952814) 3952814..3954094 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3950546..3950755 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T167620 WP_001297643.1 NZ_CP058831:c3950347-3950060 [Shigella flexneri]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T167620 NZ_CP075192:c2476349-2476053 [Ferribacterium limneticum]
ATGTATTCGATCAAAACCCTGCCTGAATTTACCGAGTGGCTAAGCGCTCTGAAGTACAAGTCGACGCTGATCCGCTTGGT
CCGTCGGTTGGACAAGGTTCAGCGTGGCAATCTTGGCGATGTGAAGGCGGTCGGTCATGGCGTATTCGAGATGCGTGAGC
ATTTCGGGCCGGGCTGGCGGATGTATTACGTGCAGCGCGGAGCGTTTTTGATTGTGATGCTGGGCGGTGGCGAAAAATCG
ACGCAGGCCGCCGATCTAGCCCGAGCCGTGAAGTTGGCGGCGACGATCAAGGAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT167620 WP_000063156.1 NZ_CP058831:c3950073-3949771 [Shigella flexneri]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT167620 NZ_CP075192:c2476047-2475742 [Ferribacterium limneticum]
ATGACCAAGAAAATTGACCCTGTGGGTCTGCCCGAATTTGATCTGGCCGAATATCTGGACAGCGAAGTGGCGATTTCGGA
GTACCTGAACCAGACTATTGCCGATGGCGACATGGCTGAGCTCGCCCACGCCCTGGGTGTGGCGGCGCGCGCTCGTGGCA
TGACCGAGGTGGCCAAGGCTTCCGGTATCACCCGGGAGGCGCTCTACAAGGCGCTGCGCCCGAATGCCAGCCCGCGTTTT
GAAACCGTCAGCAAGGTTTGTGCGGCACTCGGTGTTCGTCTGGTGGCGCAGCCGATCAATTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References