Detailed information of TA system TA167616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2340324..2340962
• Replicon: chromosome
• Accession: NZ_CP058831
• Organism: Shigella flexneri strain STLE4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: HZT21_RS11335
• Protein ID: WP_001447010.1
• Coordinates: 2340786..2340962 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HZT21_RS11330
• Protein ID: WP_001270286.1
• Coordinates: 2340324..2340740 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT21_RS11310 (2335476)	2335476..2336417	-	942	WP_001251304.1	ABC transporter permease	-
HZT21_RS11315 (2336418)	2336418..2337431	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
HZT21_RS11320 (2337449)	2337449..2338594	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
HZT21_RS11325 (2338839)	2338839..2340245	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HZT21_RS11330 (2340324)	2340324..2340740	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HZT21_RS11335 (2340786)	2340786..2340962	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HZT21_RS11340 (2341184)	2341184..2341414	+	231	WP_000494244.1	YncJ family protein	-
HZT21_RS11345 (2341506)	2341506..2343467	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HZT21_RS11350 (2343540)	2343540..2344076	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HZT21_RS11355 (2344168)	2344168..2345343	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T167616 WP_001447010.1 NZ_CP058831:c2340962-2340786 [Shigella flexneri]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T167616 NZ_CP075192:994999-995247 [Ferribacterium limneticum]
ATGCTGAAGAACCCGAAAGCTGGTGATGTAATCGAAGGGACTGGCGGTCTGCGCAAACTGCGTCATGGCGATCCACGCCG
TGGCAAAGGTAAGCGCGGTGGGTTGCGGGTGATTTACTACTGGTGGGATGGTGGCCGGCAATTCTGGCTATTCACCCTGT
ACGACAAGGATGAAATGGAGAACTTGAGTGCTGACGAAAAGAAGGCACTCAGGGGAATGTTGAAAGCTGAGCTGGAGGCC
AGACGATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT167616 WP_001270286.1 NZ_CP058831:c2340740-2340324 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT167616 NZ_CP075192:995244-995558 [Ferribacterium limneticum]
ATGAAAAAGCGGAACTTGTTTGCGGAGATTGCCGAGGGCTTCGACGCCTTGGCGAATGAGCGCACCGGCAAGCAGACGCT
GCGCACGCATAAGGTCGAGGTTCTGCCATTGGCGGAGGTTACGGCAGAGGAGCTGGTCTCGCTGCGTGAGCGCCTGCATC
TGTCGCGCCCGGTGTTTGCTGGCTACCTGCGTACCAACCCGAGAACCTTGGAGAACTGGGAGCAGGGCAGGGCCAAGCCG
AACGCCCAAGCCTCGCTACTGATTCGCCTGGTCGAGAAATTCCCGGATACGGTTGAGCGGCTTGCCGCGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8