Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 820140..820794 Replicon chromosome
Accession NZ_CP058831
Organism Shigella flexneri strain STLE4

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HZT21_RS04025 Protein ID WP_000244777.1
Coordinates 820387..820794 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HZT21_RS04020 Protein ID WP_000354046.1
Coordinates 820140..820406 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT21_RS03995 (815309) 815309..816052 + 744 WP_000951962.1 SDR family oxidoreductase -
HZT21_RS04000 (816109) 816109..817542 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
HZT21_RS04005 (817587) 817587..817898 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HZT21_RS04010 (818062) 818062..818721 + 660 WP_000250274.1 hemolysin III family protein -
HZT21_RS04015 (818917) 818917..819897 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HZT21_RS04020 (820140) 820140..820406 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HZT21_RS04025 (820387) 820387..820794 + 408 WP_000244777.1 protein YgfX Toxin
HZT21_RS04030 (820834) 820834..821355 - 522 WP_001055874.1 flavodoxin FldB -
HZT21_RS04035 (821467) 821467..822363 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HZT21_RS04040 (822388) 822388..823098 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HZT21_RS04045 (823104) 823104..824837 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T167603 WP_000244777.1 NZ_CP058831:820387-820794 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T167603 NZ_CP075189:c2478481-2478110 [Ferribacterium limneticum]
ATGATCTTGGTCGATACCTCGATCTGGATTGACCATTTACGCCAGCGTGACGAGCGCCTGAGCAAACTGCTCAATCAGGG
CCAAGTGCTGGCCCATCCTTTCGTGATCGGCGAATTAGCCTTGGGTAGCCTGCAAAACCGCACGGCGATCCTCGGCGCCC
TGCAGGATTTGCCGCAAGCCACGGTCGCCACGGAAGGCGAAGTACTGAGTTTCATCGAGCAAAATGCCCTCTACGGTATT
GGCATCGGCTACATCGACGCGCATTTGCTCGCAGCAGTGCGCCTCTCGCCGGGCGCCACATTGTGGACGCGTGATAAACG
GCTGCTGGCCGCCGGTACGGGCTTGGGGTTGGTGGAAAACGCGGTGCATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT167603 WP_000354046.1 NZ_CP058831:820140-820406 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT167603 NZ_CP075189:c2478675-2478478 [Ferribacterium limneticum]
ATGCGCACGACGCTCGCCCTCGACGATGATTTGCTTGCCAAGGCCCATGCCCTGACAGGTGTTTCGGAAAAAACCGCCTT
GATCCGCGAAGCCCTGCGCGCGCTGATCCAGCGCGAAAGCGCCAAGCGCCTGGCGCTGCTCGGGGGATCCGAGCCGCAAC
TCGAGGACGCACCAAGGCGCCAGACGGAGCCGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References