Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 95657..96455 Replicon plasmid unnamed1
Accession NZ_CP058829
Organism Shigella flexneri strain STLE3

Toxin (Protein)


Gene name ataT Uniprot ID A0A2L1KTV6
Locus tag HZT20_RS23360 Protein ID WP_023135659.1
Coordinates 95934..96455 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A141BRA2
Locus tag HZT20_RS23355 Protein ID WP_001711191.1
Coordinates 95657..95926 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT20_RS23330 (HZT20_23160) 91084..92424 - 1341 WP_023135655.1 DnaB-like helicase C-terminal domain-containing protein -
HZT20_RS23335 (HZT20_23165) 92485..93210 - 726 WP_023135656.1 hypothetical protein -
HZT20_RS23340 (HZT20_23170) 93493..94260 + 768 WP_001711193.1 hypothetical protein -
HZT20_RS23345 (HZT20_23175) 94313..94666 - 354 WP_161953766.1 hypothetical protein -
HZT20_RS23350 (HZT20_23180) 94672..95337 - 666 WP_023135658.1 AAA family ATPase -
HZT20_RS23355 (HZT20_23185) 95657..95926 + 270 WP_001711191.1 DUF1778 domain-containing protein Antitoxin
HZT20_RS23360 (HZT20_23190) 95934..96455 + 522 WP_023135659.1 GNAT family N-acetyltransferase Toxin
HZT20_RS23365 (HZT20_23195) 96624..96875 - 252 WP_023135660.1 hypothetical protein -
HZT20_RS23370 (HZT20_23200) 96878..97645 - 768 WP_023135661.1 hypothetical protein -
HZT20_RS23375 (HZT20_23205) 97668..99242 - 1575 WP_095526113.1 hypothetical protein -
HZT20_RS23380 (HZT20_23210) 99276..100532 - 1257 WP_023135663.1 phage terminase large subunit -
HZT20_RS23385 (HZT20_23215) 100535..101176 - 642 WP_023135664.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1A / tet(A) / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 - 1..169932 169932


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(48-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19382.22 Da        Isoelectric Point: 8.2535

>T167598 WP_023135659.1 NZ_CP058829:95934-96455 [Shigella flexneri]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRHVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T167598 NZ_CP075185:3063957-3064139 [Dechloromonas denitrificans]
ATGAACTCACAAAAGCTGATCCGGATGCTGGAAGACGATGGCTGGAGACAAGCGCGTGTAGCAGGTAGTCACCATCACTT
CAAACACCCGACAAAGCCGGGCCTTGTGACGGTTCCACACCCGAAGAAGGACTTGCCTGTTGGAACTGTGAACAGCATCC
TGAAACAGGCCGGGCTCAAATGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10054.53 Da        Isoelectric Point: 8.7353

>AT167598 WP_001711191.1 NZ_CP058829:95657-95926 [Shigella flexneri]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Download         Length: 270 bp

>AT167598 NZ_CP075185:3064226-3064624 [Dechloromonas denitrificans]
ATGGAATTTCCCATCGCAATCCATAAGGATGATGGGAGCGTCTACGGTGTAACTGTTCCGGACATTCCGGGCTGCCATTC
ATGGGGCGATTCCATCAACGAAGCAATTCGCAATGCGAAAGATGCAATCTATAGCCACATCGAGACCCTGCTTGAATTAG
GTGAACAAGCGGACTTCTCGGCATCAACGATTGATGACCTGACCGGTAAGGAAGATTTCGTTGGGGCAATCTGGGCTTTG
GTCGAAGTGGATTTTTCAGAACTCGACCCTAAGCCTGAGCGCGTCAACATCAGCCTTCCGCGGTTTGTTCTTCGTCGGAT
TGATGAGTACACAAAAGCTCACCATGAAACCCGAAGTGGTTTCCTGGCAAGGGCAGCAATGCATGCACTAGCAAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1KTV6

Antitoxin

Source ID Structure
AlphaFold DB A0A141BRA2

References