Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4055863..4056439 Replicon chromosome
Accession NZ_CP058828
Organism Shigella flexneri strain STLE3

Toxin (Protein)


Gene name shpA Uniprot ID A0A0E2LC92
Locus tag HZT20_RS19445 Protein ID WP_001299718.1
Coordinates 4056152..4056439 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0E2KXJ8
Locus tag HZT20_RS19440 Protein ID WP_000063159.1
Coordinates 4055863..4056165 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT20_RS19425 (4052452) 4052452..4054602 + 2151 WP_001299714.1 pyruvate/proton symporter BtsT -
HZT20_RS19430 (4054652) 4054652..4054855 + 204 WP_000467859.1 YbdD/YjiX family protein -
HZT20_RS19435 (4054866) 4054866..4055822 + 957 WP_001299717.1 GTPase -
HZT20_RS19440 (4055863) 4055863..4056165 - 303 WP_000063159.1 BrnA antitoxin family protein Antitoxin
HZT20_RS19445 (4056152) 4056152..4056439 - 288 WP_001299718.1 BrnT family toxin Toxin
HZT20_RS19450 (4056636) 4056636..4057802 + 1167 Protein_3818 restriction endonuclease -
HZT20_RS19455 (4057866) 4057866..4059485 + 1620 WP_001029740.1 class I SAM-dependent DNA methyltransferase -
HZT20_RS19460 (4059475) 4059475..4060866 + 1392 WP_000535334.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.60 Da        Isoelectric Point: 7.4697

>T167595 WP_001299718.1 NZ_CP058828:c4056439-4056152 [Shigella flexneri]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T167595 NZ_CP075183:c3311226-3309904 [Hydrogenophaga taeniospiralis]
ATGGATCGTCAGCTCGAGGTCTGGTTGTTTGGCAACCCCATCGGCACGCTCACCCAGGCCGACGGCAGGCTCGCCTTCAG
CTACCGACCCGAGTGGCTGGCCGGTGGGCAGGCCACGCCGCTGTCCCAGTCCCTGCCGCTGCAGGCAGAAGCGTTTGACG
ACCGCGCCACGCGCCCCTTCTTTGCCGGCCTCTTGCCCGAGGGCGACAAGCGCCGCCTCGTGGCCCAGGCCCTGGGCGTC
TCCCGCCAGAACGACTTTGCCCTGCTCGACGGCATTGGCGGCGAATGCGCCGGCGCTGTCACCCTGCTGGAACCCGGCCA
AACGCCCAGCCATGCCGCCCCGGCCAATGCCGTGCGCTGGCTGGACGATGCAGACCTGCTCAAGCTGCTGGCTGAAATGC
CCCGCCGCCCCATGCTTGCGGGCGACGGCGACCTGCGTCTCTCCCTCGCCGGAGCACAAGACAAGCTGCCCGTGGTGGTG
GCGGGCGAGCGCATCGGTCTGCCCCTGTTCGGCTCCCCCAGCACCCACATCCTCAAGCCCGCCATCGAAGGCATTGAAGG
CAGTGTCTTCAACGAAGGCTTCTGCATGGCGCTCGCGCGGGCCATGGGGCTCGACGCCGCGCCCAGCGAGATCCGCCGGG
TGCAGGGCCAGCCCTACCTGCTGGTGCAGCGGTACGACCGCCTGCCAGTTGAGCAATCGCCTGCGGCAACCATCCAGCGC
CTGCACCAGGAAGACTTTTGCCAGGCCCTGGGCGTGCCGCCCGAACTGAAGTACCAGAACGAAGGTGGCCCCGACCTCGC
CCAAGCCTTTGCCCTGGTGCGCAGCGCCACCCGCCCCAATGCGCCGCACATCCTCAAACTGCTCGGCTTCGTGCTGTTCA
ACGCCCTCATCGGCAACCACGACGCCCACGCCAAAAACTTTTCGTTGCTCTACTCCCCGCGCTGCGCTGCGCTCGCGCCG
CTTTACGACGCACTCTCCACCGCCGTCTACCCGGATCTCACCGACAAGATGGCCATGAAGATCGGCAGCAAGTACCGCTT
TGCCGAGCTGCATGCCCGCCACTGGGAACAGTTCGCCACCAGCGCTGGCCTGTCACCGGCCCAGGTGAAGAAAGAACTCA
AGCGGTTTGCCGAGCGCCTGCCCGACCAAGTTCGCCTTGTTCGCGCGGAATTCCTGGTCACGGATCTCTTGCACCCGGTG
CTCAGCCAGATCAGCGAGCTGATCGAAGACCGCTGCGCCACCACCTTGCGCCGCCTCGCCATGTCCCCGGCGCAAGCCGA
GGCCGAAACCGACACCCCAGAAGAGGAAGGTCCCGCACCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11536.01 Da        Isoelectric Point: 9.4646

>AT167595 WP_000063159.1 NZ_CP058828:c4056165-4055863 [Shigella flexneri]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEEIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT167595 NZ_CP075183:c3311464-3311228 [Hydrogenophaga taeniospiralis]
ATGTCCACCCGCATCGACACCCCCGAAGCCCTCGGCCAAGCCGTGCGCGGCGCCCGCAAGCAGCTGAACCTCACCCAGCC
CCAGCTCGCGCTGGCGGCGGGTGTCGGTGTGCGCTTCATCGTGGACCTCGAAGCCGGCAAGCCCACCTTGCGGCTGGAGA
ACATCCTGCGCGTGCTGCACGCCCTGGGTGGCGAACTGCAAGTCGATGGCCTGCCCACCCCGCGTGAGGGCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2LC92

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2KXJ8

References