Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3521019..3521637 Replicon chromosome
Accession NZ_CP058828
Organism Shigella flexneri strain STLE3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HZT20_RS16930 Protein ID WP_001291435.1
Coordinates 3521419..3521637 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HZT20_RS16925 Protein ID WP_000344800.1
Coordinates 3521019..3521393 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT20_RS16915 (3516108) 3516108..3517301 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HZT20_RS16920 (3517324) 3517324..3520473 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HZT20_RS16925 (3521019) 3521019..3521393 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HZT20_RS16930 (3521419) 3521419..3521637 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HZT20_RS16935 (3521809) 3521809..3522360 + 552 WP_000102564.1 maltose O-acetyltransferase -
HZT20_RS16940 (3522476) 3522476..3522946 + 471 WP_000136192.1 YlaC family protein -
HZT20_RS16945 (3523110) 3523110..3524660 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HZT20_RS16950 (3524702) 3524702..3525055 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HZT20_RS16960 (3525434) 3525434..3525745 + 312 WP_000409911.1 MGMT family protein -
HZT20_RS16965 (3525776) 3525776..3526348 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T167593 WP_001291435.1 NZ_CP058828:3521419-3521637 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T167593 NZ_CP075183:2817209-2817478 [Hydrogenophaga taeniospiralis]
ATGTCGATCCAGTCGTTCGCCTGCTCCGACACGGAGAGTCTGTTCACCACAGGCAAGTGTCGCCGGTTCGTCAACATCAA
GAGCGTAGCCGAGCGCAAATTGGCCCAGCTCGACGCGGCGCCTTCGGTGTGTTTCATGAAGGCCCCGCCAGGCAACGACC
TGAAGGAATACGACGGTGCCTGGCATGTGCGAATCAACGACCAGTGGCGACTGACCTTCAAGTGGGGTGAGAGCGGACCC
TATGACGTGCTCATCGAAGACCCACACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT167593 WP_000344800.1 NZ_CP058828:3521019-3521393 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT167593 NZ_CP075183:2817526-2817831 [Hydrogenophaga taeniospiralis]
ATGTTCAAGAACGGTATGCGTCCTGTCCACCCAGGTGAGATTTTGCTGGAGGACTACATCAAACCCATGGGCGTCAGCGT
GCGCGCCGTGGCCATGGCCCTGCATGTGCCTTACTCGCGCCTGAGCGAAATCACCAAGGGCGAGCGCGGCGTGTCGGCTG
ACACGGCCCTGCGCCTGGAGCGCTACTTCGGCAGCGAGGCCAAGGGCTGGTTGAACCTTCAGGCAGCCTACGAGTTGCGA
ATGGCCGAGATCGAGAACGGCAAAGCCATCGCCAAAGAGATCCAGCCGTTTGCGCTGGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References