Detailed information of TA system TA167583

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1845062..1845894
• Replicon: chromosome
• Accession: NZ_CP058828
• Organism: Shigella flexneri strain STLE3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: HZT20_RS08640
• Protein ID: WP_000854765.1
• Coordinates: 1845062..1845436 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: HZT20_RS08645
• Protein ID: WP_001295723.1
• Coordinates: 1845526..1845894 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT20_RS08605 (1840521)	1840521..1840850	+	330	WP_000450409.1	DUF496 family protein	-
HZT20_RS08610 (1840951)	1840951..1841217	-	267	WP_001360325.1	EutP/PduV family microcompartment system protein	-
HZT20_RS08615 (1841407)	1841407..1842189	-	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
HZT20_RS08620 (1842186)	1842186..1843208	-	1023	WP_000255946.1	IS21-like element IS100 family transposase	-
HZT20_RS08625 (1843546)	1843546..1843935	-	390	WP_077249349.1	transposase	-
HZT20_RS08630 (1844733)	1844733..1844813	-	81	Protein_1696	hypothetical protein	-
HZT20_RS08635 (1844859)	1844859..1845062	-	204	WP_001398797.1	DUF5983 family protein	-
HZT20_RS08640 (1845062)	1845062..1845436	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
HZT20_RS08645 (1845526)	1845526..1845894	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HZT20_RS08650 (1846057)	1846057..1846278	-	222	WP_000692345.1	DUF987 domain-containing protein	-
HZT20_RS08655 (1846286)	1846286..1846474	+	189	Protein_1701	IS4 family transposase	-
HZT20_RS08660 (1846453)	1846453..1846644	+	192	Protein_1702	cobalamin biosynthesis protein	-
HZT20_RS08665 (1846778)	1846778..1847323	+	546	WP_000973159.1	bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase	-
HZT20_RS08670 (1847320)	1847320..1848063	+	744	WP_001297350.1	adenosylcobinamide-GDP ribazoletransferase	-
HZT20_RS08675 (1848075)	1848075..1849154	+	1080	Protein_1705	nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase	-
HZT20_RS08680 (1849216)	1849216..1850151	+	936	WP_000986331.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T167583 WP_000854765.1 NZ_CP058828:c1845436-1845062 [Shigella flexneri]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T167583 NZ_CP075174:3962982-3963932 [Salmonella enterica subsp. houtenae serovar 45:g,z51:-]
ATGGATAATTTAACGCTACTTTTTTCTGATGTGGCGGATGCTCTTGGTATTGAATCCGCCGCTATTGTAGAAAAAGATCA
CTATATCGTTGAGCTTCTACGTTTACTGAAACCGCTAACATTTGACACTCATCAACTGGTGTTCGCTGGGGGAACTGCAC
TGGCAAAATCGGGCATTGTGCTTAACCGAATGTCGGAAGACGTAGATATAAAACTGGTACCCACTGCCACTTTCCAGACT
CTTTCGCGCACCCAGCGCAAAGGTATTCGTAAAGAGATTGTCCAGACAATTTCTAGTGCAACAACTGAAACGGGCATGTT
CACCTTTGACGATACCCACCCAAAAGTAACGCGGGATGAATATCGTTATAACGACATACCCGTTCGCTATCCGCAACGGG
TAGCGCAAGCACCTTGCCTGCGCCCTTTTATCCGGCTGGAATTGATGGAAGCAAATTTGCTTGAGGCACCAGAGTTGAGG
GATATTCATTCACTGGTGACGGCACTTACTGGAAAAACACCTGTCATTTCGGCCTTCCCCTGCGCCACGATTACCAGTAC
CCAGGCAGAAAAACTGGTGGCAATGCTACGCCGTACGGCAGCAGTGGCGCGTAATGTTGAGCGCCTGGATGATGCTTCGC
TGGTACGTCATATCTACGACACATACTGTATCGCACAGACAGGTAATGTTGATATGCGTTTACTTACTGATTTTTTTAAG
CGAACCATTGAGCAGGATATCCAGCGTTATGGCAACCAGTATCCGCAGTTTTGTGTTTCACCAAATGATGAGCTAAAAAT
GGGGCTGGACGAACTTGAGCGTAACCCGCTTTATAAGACACGCTACCAGCAATTTGTTGCCCCAATGGTATATGGTGCTC
GCAAGGTATCGTGGGATGAAGCGTATGCTTCCTTTCGCCAACTGGCGCTAGCTATCCTGAATGCTAGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT167583 WP_001295723.1 NZ_CP058828:c1845894-1845526 [Shigella flexneri]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT167583 NZ_CP075174:3962594-3962989 [Salmonella enterica subsp. houtenae serovar 45:g,z51:-]
ATGACGATAAAAACACGCATTCAGTCACGACTGAAACGCTCAAAACGCTATGTATTCACTCGTGACGATTTCAAAGATAT
TGCAGGCTATGATCAGATTGGCAGAGTATTGCGCGACCTTCTCAAAGAGGGGCAACTACTGAAGGTGGGCTACGGTGTGT
ACACCAAAGCGCGTCGAAACAGCATTACTGGAAAAGTCATGCCCGCCGCGCCAGGGGGATCTTCAGCAGTGCTCGTTGAA
ACGCTGGATCGCCTGAAGGTACGTTACCGCTATGTGGGAGCTACACAGGCATATAATAGTGGCGAATCTACGCAAATCCC
TGTGTCACCAGAGATCAAAACATCGCCGCGCTTTAAACGCGCGTTATCTGTGGGGAACAGCAAACTAAATGGATAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52