Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4025915..4026412 Replicon chromosome
Accession NZ_CP058826
Organism Shigella flexneri strain STLE1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HZT18_RS19680 Protein ID WP_001173343.1
Coordinates 4025915..4026088 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HZT18_RS19685 Protein ID WP_000398619.1
Coordinates 4026116..4026412 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT18_RS19670 (4022585) 4022585..4024042 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HZT18_RS19675 (4024279) 4024279..4025796 + 1518 WP_001295074.1 lysine--tRNA ligase -
HZT18_RS19680 (4025915) 4025915..4026088 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HZT18_RS19685 (4026116) 4026116..4026412 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HZT18_RS19690 (4026639) 4026639..4026911 - 273 WP_096837934.1 GNAT family N-acetyltransferase -
HZT18_RS19695 (4026923) 4026923..4027153 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
HZT18_RS19700 (4027334) 4027334..4028965 + 1632 WP_001216477.1 sensor histidine kinase -
HZT18_RS19705 (4028962) 4028962..4029681 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T167575 WP_001173343.1 NZ_CP058826:c4026088-4025915 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T167575 NZ_CP075174:789088-789501 [Salmonella enterica subsp. houtenae serovar 45:g,z51:-]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTACTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCATTGTGGATGATGCTACTGTCGCTGGTCGTGTTTGACTGCG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAAATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCCCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCTGAGTCTGGACGCCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATAATGTTACAGCAACCGATAT
CGCGCCAACACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT167575 WP_000398619.1 NZ_CP058826:c4026412-4026116 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT167575 NZ_CP075174:788841-789107 [Salmonella enterica subsp. houtenae serovar 45:g,z51:-]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTGATGAATCACGGTAAACCGGCCGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References