Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3817073..3817649 Replicon chromosome
Accession NZ_CP058826
Organism Shigella flexneri strain STLE1

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HZT18_RS18615 Protein ID WP_001297643.1
Coordinates 3817362..3817649 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag HZT18_RS18610 Protein ID WP_000063160.1
Coordinates 3817073..3817375 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT18_RS18595 (3813669) 3813669..3815819 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
HZT18_RS18600 (3815869) 3815869..3816072 + 204 WP_000467859.1 YbdD/YjiX family protein -
HZT18_RS18605 (3816083) 3816083..3817039 + 957 WP_001297640.1 GTPase -
HZT18_RS18610 (3817073) 3817073..3817375 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
HZT18_RS18615 (3817362) 3817362..3817649 - 288 WP_001297643.1 BrnT family toxin Toxin
HZT18_RS22320 (3817848) 3817848..3818544 - 697 Protein_3653 IS1 family transposase -
HZT18_RS18630 (3818859) 3818859..3820091 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
HZT18_RS18635 (3820132) 3820132..3821412 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3817848..3818057 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T167573 WP_001297643.1 NZ_CP058826:c3817649-3817362 [Shigella flexneri]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T167573 NZ_CP075171:c3570241-3570014 [Leptodesmis sichuanensis PKUAC-SCTA121]
ATGAAGATTTGGGTGGATGCTCAACTACCTCCAACTCTGGCAAATTGGTTGTCAGAATCCTTTGGCTTAGAAGCTTATGC
TTTGCGAGACTTATCACTTCGAGATGCCAAGGATATCGAAATTTTTGAGGCGGCACGAGCGGAAAACGCTGTGATTAGGA
GCTTGCAAATAAATAAAATACCAGTCATTTTAGCGGGAGCAGAGCGGAAATATCCCTCTCCTGGCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT167573 WP_000063160.1 NZ_CP058826:c3817375-3817073 [Shigella flexneri]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT167573 NZ_CP075171:c3570471-3570238 [Leptodesmis sichuanensis PKUAC-SCTA121]
ATGAATTCAGCAACTGGCTTGTTAACTCGCATTACTCAAACTCCTGGTCAGTGCGGTGGTCGTCCCTGCATTAGAGGGAT
GCGAATTCGAGTTACCGATATCTTGGAAATGCTGGCTGAAAACGTGAGTGTTAATGAAATTTTAGAAGATTTTCCCGACT
TAGAACTAGCAGATATTCAGGCTTGCTTGCTATTTGCAGCACGACGTACTGATTTTCCTAGACTAACAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References