Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3286535..3287153 Replicon chromosome
Accession NZ_CP058826
Organism Shigella flexneri strain STLE1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HZT18_RS16035 Protein ID WP_001291435.1
Coordinates 3286935..3287153 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HZT18_RS16030 Protein ID WP_000344800.1
Coordinates 3286535..3286909 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT18_RS16020 (3281624) 3281624..3282817 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HZT18_RS16025 (3282840) 3282840..3285989 + 3150 WP_240764643.1 efflux RND transporter permease AcrB -
HZT18_RS16030 (3286535) 3286535..3286909 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HZT18_RS16035 (3286935) 3286935..3287153 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HZT18_RS16040 (3287325) 3287325..3287876 + 552 WP_000102564.1 maltose O-acetyltransferase -
HZT18_RS16045 (3287992) 3287992..3288462 + 471 WP_000136192.1 YlaC family protein -
HZT18_RS16050 (3288626) 3288626..3290176 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HZT18_RS16055 (3290218) 3290218..3290550 - 333 WP_001741293.1 DUF1428 family protein -
HZT18_RS16065 (3290950) 3290950..3291261 + 312 WP_000409911.1 MGMT family protein -
HZT18_RS16070 (3291292) 3291292..3291864 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T167569 WP_001291435.1 NZ_CP058826:3286935-3287153 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T167569 NZ_CP075171:358285-358560 [Leptodesmis sichuanensis PKUAC-SCTA121]
ATGGAATACGAATGGGATGAAGCAAAGCGTCTCGCCAATCTTCGTAAGCATGGAATTGACTTTATTGATGTCCCAGCCAT
ATTTGGTGGCGACACTGTAACTGTTGAGGATGATCGCTATAGCTATGGGGAGCAGCGGTTTATCACATTTGGCTTGTTGC
AAGGGCGAGTGATTGCTGTTGTCCATACCGAACGAGAGGATTATACCCGTATTATTTCCGCAAGAAAGGCAACGAAATAT
GAACAGCGAGCCTACTTCGAGCAACTCTCAAACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT167569 WP_000344800.1 NZ_CP058826:3286535-3286909 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT167569 NZ_CP075171:358580-358801 [Leptodesmis sichuanensis PKUAC-SCTA121]
ATGACGGACGAAGATATTGATTTCTCAGACTGCCCAGAAGTTACACCAGAGATGTTTGCAAAAGCAATAGTGCGACGTGG
TTTACCCGTTGCAAAAGCAAAGACCCAGGTTACACTCCGTATCGATAGCGATGTTTTGGATTGGTTTAAGTCTCAGGGAC
GTGGCTATCAAACTCAAATCAATCAGTTGTTGCGGGCTTATATGGAAGCACATCAGCGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References