Detailed information of TA system TA167568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 2887610..2888315
• Replicon: chromosome
• Accession: NZ_CP058826
• Organism: Shigella flexneri strain STLE1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HZT18_RS14100
• Protein ID: WP_000539521.1
• Coordinates: 2887610..2887996 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HZT18_RS14105
• Protein ID: WP_001280945.1
• Coordinates: 2887986..2888315 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT18_RS14080 (2883614)	2883614..2884240	+	627	WP_001295292.1	glutathione S-transferase GstB	-
HZT18_RS14085 (2884237)	2884237..2885352	-	1116	Protein_2769	aldose sugar dehydrogenase YliI	-
HZT18_RS14090 (2885463)	2885463..2885846	-	384	WP_000497137.1	biofilm formation regulator BssR	-
HZT18_RS14095 (2886059)	2886059..2887384	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HZT18_RS14100 (2887610)	2887610..2887996	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HZT18_RS14105 (2887986)	2887986..2888315	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HZT18_RS14110 (2888385)	2888385..2889713	-	1329	WP_000086877.1	GGDEF domain-containing protein	-
HZT18_RS14115 (2889721)	2889721..2892069	-	2349	WP_001338403.1	EAL domain-containing protein	-
HZT18_RS14120 (2892247)	2892247..2893158	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T167568 WP_000539521.1 NZ_CP058826:2887610-2887996 [Shigella flexneri]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T167568 NZ_CP075171:c350661-350284 [Leptodesmis sichuanensis PKUAC-SCTA121]
ATGAACGCATTGCCAAAGCCAGTTGAATGGGTCGGAAGCTCTCTGGAAGATTTGAAGGAGTTTCCGGAGGATGTGCGGCA
AACGGTTGGGTACGCACTGTACTTGGCCCAATGTGGTGAAAAGCACCCCTCAGCCAAGCCGCTCAAAGGATTTAGGGGAA
CTGGGGTACTGGAAATTGTAGAAAATTTTGACGGAGATACTTATCGAGCCGTCTACACGGTAAAACTGGCTGGAGTGGTC
TACGTCTTACACGCTTTCCAGAAAAAATCAAAGCAAGGTATTGCTACGCCTAAGCAGGATATTGACCTGATCGAAGCAAG
ACTGAAGCGAGCCAAGGAACACTATTCACAAAACTACAGCAAACATCAAGGAGAGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT167568 WP_001280945.1 NZ_CP058826:2887986-2888315 [Shigella flexneri]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT167568 NZ_CP075171:c350281-349958 [Leptodesmis sichuanensis PKUAC-SCTA121]
ATGACTGAAGAAATTGGAGTACAAGCCAGTAGCGGCAACGTGTTTGCAGATTTGAGTTTGGAAAATGCTGACGAATTGCT
GGTTAAAGCAGAACTTGCTCGGCGGATCAGCAGTATCATTACAACTCAACAAATGACCCAAATTGAGGCAGCAGAGGCGT
TAGGAATTGACCAACCTAAAATCTCGGCATTAATCAATGGAAAATTAGCAGGTTTTTCGACCGCACGATTGTTTCGCTTT
CTCAATGCTTTGGGGCGAGATGTGGAAATTGTAGTGAAACCCAAATCTCATGCACAAGCTCAAACGCGCGTTGTTGCTTC
GTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6