Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 868024..868678 Replicon chromosome
Accession NZ_CP058826
Organism Shigella flexneri strain STLE1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HZT18_RS04235 Protein ID WP_000244777.1
Coordinates 868271..868678 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HZT18_RS04230 Protein ID WP_000354046.1
Coordinates 868024..868290 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT18_RS04205 (863224) 863224..863921 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
HZT18_RS04210 (863993) 863993..865426 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
HZT18_RS04215 (865471) 865471..865782 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HZT18_RS04220 (865946) 865946..866605 + 660 WP_000250274.1 hemolysin III family protein -
HZT18_RS04225 (866801) 866801..867781 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HZT18_RS04230 (868024) 868024..868290 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HZT18_RS04235 (868271) 868271..868678 + 408 WP_000244777.1 protein YgfX Toxin
HZT18_RS04240 (868718) 868718..869239 - 522 WP_001055874.1 flavodoxin FldB -
HZT18_RS04245 (869351) 869351..870247 + 897 WP_000806639.1 site-specific tyrosine recombinase XerD -
HZT18_RS04250 (870272) 870272..870982 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HZT18_RS04255 (870988) 870988..872721 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T167556 WP_000244777.1 NZ_CP058826:868271-868678 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T167556 NZ_CP075169:2516485-2516973 [Sodalis ligni]
GTGATCTTGGCCCCTGAGCCCCTTCACGTCGGGCATATTCTCTCCTCATTTTGTTGCGGCGTGGATTCTATGGACAACTG
GCTAAAACAGAGGGCGATGAAAAATCAGCTAACCGGCGCATCCCGGACGTTCGTCTGCTGCGACGACTTGAAGGTGATGG
CGTATTACTCGTTGGCGTCAAGCGCGGTGATGACAACTGCCGCGCCTGGACGCTTTCGCCGTAATATGCCTGATCCAATC
CCGGTGGTAGTGCTAGGCCGTTTGGCGGTAGATACATCATTGCACGGTCAGGGCGTCGGGCGGGCACTGGTGCGGGATGC
CGGACTACGGGTGATTCAGGTGGCGGAAACCATCGGTATTCGCGGGGTGTTGGTTCATGCGCTGTCTACAGAGGCGCGGG
AATTTTACCTGAGGGTCGGGTTTGAACCTTCGCCGATTGACCCGATGATGCTGATGGTGACGTTGGGGGACTTGGTGGAG
AGCGTTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT167556 WP_000354046.1 NZ_CP058826:868024-868290 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT167556 NZ_CP075169:2516195-2516488 [Sodalis ligni]
ATGCCAGCGACGAACGGCGTTTCCGTCAAACGACAAACATTGAATTTACGCATCAAACCCGCCGAGCGCGATCTTATCGA
TCGTGCGGCGAAGGCTAAAGGGAAAAACCGTACTGACTTTGTACTGGAGGCCGCGCACGCTGCGGCAGAGGACGTGCTGA
TCGAGCAGCGCATCATCATGGCGGACCCGGACGCCTATCAGGAATTTCTTGTCCGTCTGGATCAAGCTCCTGCACCCAAC
GCCGCGCTGCGTAAAACCATGCAAACGCCCGCGCCGTGGGAACAGAAAAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References