Detailed information of TA system TA167549

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3773115..3773794
• Replicon: chromosome
• Accession: NZ_CP058796
• Organism: Shigella flexneri strain STLIN_8

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HZT12_RS18770
• Protein ID: WP_016246873.1
• Coordinates: 3773453..3773794 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: -
• Locus tag: HZT12_RS18765
• Protein ID: WP_016246874.1
• Coordinates: 3773115..3773432 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT12_RS18720 (3768553)	3768553..3769374	+	822	WP_001547762.1	DUF932 domain-containing protein	-
HZT12_RS18725 (3769591)	3769591..3770292	+	702	WP_001581462.1	WYL domain-containing protein	-
HZT12_RS18730 (3770333)	3770333..3770569	+	237	WP_001144031.1	protein YpjK	-
HZT12_RS18735 (3770569)	3770569..3771012	+	444	WP_000649865.1	hypothetical protein	-
HZT12_RS18740 (3771035)	3771035..3771502	+	468	WP_001547765.1	protein YkfB	-
HZT12_RS18745 (3771579)	3771579..3771818	+	240	WP_000194654.1	DUF905 family protein	-
HZT12_RS18750 (3771916)	3771916..3772374	+	459	WP_113372854.1	antirestriction protein	-
HZT12_RS18755 (3772390)	3772390..3772866	+	477	WP_000811693.1	RadC family protein	-
HZT12_RS18760 (3772875)	3772875..3773096	+	222	WP_000691994.1	DUF987 domain-containing protein	-
HZT12_RS18765 (3773115)	3773115..3773432	+	318	WP_016246874.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HZT12_RS18770 (3773453)	3773453..3773794	+	342	WP_016246873.1	TA system toxin CbtA family protein	Toxin
HZT12_RS18780 (3774366)	3774366..3775619	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
HZT12_RS18785 (3775631)	3775631..3776734	-	1104	WP_240765267.1	glutamate 5-kinase	-
HZT12_RS18790 (3777022)	3777022..3778077	+	1056	WP_112034093.1	phosphoporin PhoE	-
HZT12_RS18795 (3778116)	3778116..3778517	-	402	WP_000174677.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12987.05 Da
• Isoelectric Point: 10.2764

>T167549 WP_016246873.1 NZ_CP058796:3773453-3773794 [Shigella flexneri]
MKTLPATTQRAAKPCLAPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T167549 NZ_CP075169:c315965-315717 [Sodalis ligni]
ATGCAAGAACCGACTTGTATCACCTTATCGCCTTCATCGCTGAGCACAATCATTCCCGCTGAATCTCTACTGCAACATAT
CGAAGAATCCGTGATCCCTGCAACTGAGCACCCTTACTTATATCGCCCTGGGCGTGTGCCGGGTACCAGAGAAATAGTCG
TCATCCCTCACTACATCGTTGTTTATCGAGTACTCAGTGATTATCTCGAAGTTTTGGCTGTGCTGCATGCCAGGCAGGAA
TACCCCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11831.42 Da
• Isoelectric Point: 7.0169

>AT167549 WP_016246874.1 NZ_CP058796:3773115-3773432 [Shigella flexneri]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDVECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT167549 NZ_CP075169:c316208-315993 [Sodalis ligni]
ATTGCCGGATTAGCGTGGAAACGTTTTGTCAAAAAACAGTCCCCTATCGTGTCGGAATTCGAGACCCAAGAGCAAGCCGG
GCGTTATGAATGCTGGTTTCGCGCTAAAGTCAATACATCCATGAATGCGGCAATACCCAACGTGCCGCACAATGAGGTTA
TGGCTGGCGCACGTAAAGTTATCGAAAAATCAAATAATCGCAGGAGATTGGCTTAA