Detailed information of TA system TA167548

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3720975..3721654
• Replicon: chromosome
• Accession: NZ_CP058796
• Organism: Shigella flexneri strain STLIN_8

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: HZT12_RS18445
• Protein ID: WP_000854680.1
• Coordinates: 3721313..3721654 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: HZT12_RS18440
• Protein ID: WP_000070396.1
• Coordinates: 3720975..3721292 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT12_RS18395 (3716412)	3716412..3717233	+	822	WP_000197387.1	DUF932 domain-containing protein	-
HZT12_RS18400 (3717450)	3717450..3718151	+	702	WP_000189411.1	WYL domain-containing protein	-
HZT12_RS18405 (3718192)	3718192..3718428	+	237	WP_001144031.1	protein YpjK	-
HZT12_RS18410 (3718428)	3718428..3718872	+	445	Protein_3614	hypothetical protein	-
HZT12_RS18415 (3718895)	3718895..3719362	+	468	WP_001385283.1	protein YkfB	-
HZT12_RS18420 (3719439)	3719439..3719678	+	240	WP_000194654.1	DUF905 family protein	-
HZT12_RS18425 (3719776)	3719776..3720234	+	459	WP_000211838.1	antirestriction protein	-
HZT12_RS18430 (3720250)	3720250..3720726	+	477	WP_000811693.1	RadC family protein	-
HZT12_RS18435 (3720735)	3720735..3720956	+	222	WP_000691994.1	DUF987 domain-containing protein	-
HZT12_RS18440 (3720975)	3720975..3721292	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
HZT12_RS18445 (3721313)	3721313..3721654	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
HZT12_RS18450 (3722317)	3722317..3722529	+	213	Protein_3622	integrase core domain-containing protein	-
HZT12_RS18455 (3722571)	3722571..3723314	-	744	WP_032170462.1	AraC family transcriptional regulator	-
HZT12_RS18465 (3724575)	3724575..3724862	-	288	Protein_3625	hypothetical protein	-
HZT12_RS18470 (3725024)	3725024..3725080	+	57	WP_219351404.1	YsgD/CorL family protein	-
HZT12_RS18475 (3725232)	3725232..3726182	+	951	WP_000947148.1	magnesium/cobalt transporter CorA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3716412..3746588	30176

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T167548 WP_000854680.1 NZ_CP058796:3721313-3721654 [Shigella flexneri]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T167548 NZ_CP075146:c4607244-4607092 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT167548 WP_000070396.1 NZ_CP058796:3720975-3721292 [Shigella flexneri]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT167548 NZ_CP075146:c4607015-4606881 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7