Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3521517..3522135 Replicon chromosome
Accession NZ_CP058796
Organism Shigella flexneri strain STLIN_8

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HZT12_RS17460 Protein ID WP_001291435.1
Coordinates 3521917..3522135 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HZT12_RS17455 Protein ID WP_000344800.1
Coordinates 3521517..3521891 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT12_RS17445 (3516606) 3516606..3517799 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HZT12_RS17450 (3517822) 3517822..3520971 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HZT12_RS17455 (3521517) 3521517..3521891 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HZT12_RS17460 (3521917) 3521917..3522135 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HZT12_RS17465 (3522307) 3522307..3522858 + 552 WP_097466604.1 maltose O-acetyltransferase -
HZT12_RS17470 (3522974) 3522974..3523444 + 471 WP_000136192.1 YlaC family protein -
HZT12_RS17475 (3523608) 3523608..3525158 + 1551 WP_016241944.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HZT12_RS17480 (3525200) 3525200..3525553 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HZT12_RS17490 (3525932) 3525932..3526243 + 312 WP_000409911.1 MGMT family protein -
HZT12_RS17495 (3526274) 3526274..3526846 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T167547 WP_001291435.1 NZ_CP058796:3521917-3522135 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T167547 NZ_CP075146:c4607015-4606881 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT167547 WP_000344800.1 NZ_CP058796:3521517-3521891 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT167547 NZ_CP075146:c4607244-4607092 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References