Detailed information of TA system TA167546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3156026..3156731
• Replicon: chromosome
• Accession: NZ_CP058796
• Organism: Shigella flexneri strain STLIN_8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HZT12_RS15690
• Protein ID: WP_000539521.1
• Coordinates: 3156026..3156412 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HZT12_RS15695
• Protein ID: WP_001280945.1
• Coordinates: 3156402..3156731 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT12_RS15670 (3152030)	3152030..3152656	+	627	WP_016241992.1	glutathione S-transferase GstB	-
HZT12_RS15675 (3152653)	3152653..3153768	-	1116	WP_000555031.1	aldose sugar dehydrogenase YliI	-
HZT12_RS15680 (3153879)	3153879..3154262	-	384	WP_000497137.1	biofilm formation regulator BssR	-
HZT12_RS15685 (3154475)	3154475..3155800	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HZT12_RS15690 (3156026)	3156026..3156412	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HZT12_RS15695 (3156402)	3156402..3156731	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HZT12_RS15700 (3156801)	3156801..3157775	-	975	WP_240765210.1	GGDEF domain-containing protein	-
HZT12_RS15710 (3158597)	3158597..3158905	-	309	Protein_3084	hypothetical protein	-
HZT12_RS15715 (3158913)	3158913..3160490	-	1578	Protein_3085	cyclic diguanylate phosphodiesterase	-
HZT12_RS15720 (3160545)	3160545..3161242	+	698	WP_240765212.1	IS1-like element IS1A family transposase	-
HZT12_RS15725 (3161258)	3161258..3161476	-	219	Protein_3087	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T167546 WP_000539521.1 NZ_CP058796:3156026-3156412 [Shigella flexneri]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T167546 NZ_CP075146:c1303800-1303309 [Xanthomonas campestris pv. campestris]
ATGACGGTATCGGCGCCGGAACCGCTGACAGCGCATCACCGGGTCGAACCGTTCGCATCGGGCGTGGAGAGCCTGGATCA
CTGGCTCAAGCGCCGCGCCCTGAAAAATCAGGCAACCGGTGCGTCACGGACCTTCGTTGCCTGCGAGGATGACCGCGTGG
TGGCCTATTACGCCCTGGCGTCGAGTGCGGTCGCCGTGGACGCGACGCCCGGCCGCTTCCGTCGCAACATGCCGGACCCG
ATCCCCGTGGTGGTGCTTGGGAGGCTTGCTGTTGACCAATCCCTGCATGGCAGAGGTTTTGGCCGCGCCCTGATGCAGGA
TGCTGGCAAGCGCATCTTGCATGCGGCCGACACCATCGGCATTCGCGGGCTGCTCGTGCATGCGCTCTCGGCGGACGCCA
AGGCGTTTTACGAGCGCATCGGCTTCGAACCTTCCCCGCTGGATCCCATGGTGCTGGTCGTCACGCTGGAAGACCTGAAA
GCCAGTCTGTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT167546 WP_001280945.1 NZ_CP058796:3156402-3156731 [Shigella flexneri]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT167546 NZ_CP075146:c1304087-1303797 [Xanthomonas campestris pv. campestris]
ATGTCCACACCCAACACTACGCCTGGCAAGCGCGAAACCTTGAATCTCCGCATCAAGCCGGAGGAGCGCAGCCTGATCGA
CCGGGCCGCCAAGGCGCGCGGGAAGAACCGCACCGATTTCATGCTGGACGCAGCGCGCTCGGCGGCCGAGGAAGCCTTGC
TGGACCAGACCCTCATCACGGCCAGCCCGGACGCCTACGCCGCCTTTTTGGCACGGCTGGACATGCCGCCGCAGCCGAAC
GCGCGCCTGCGCAAGACGATGCAGACGCCCGCTCCTTGGGAGAAGGCATGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6