Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1860179..1861010 Replicon chromosome
Accession NZ_CP058796
Organism Shigella flexneri strain STLIN_8

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag HZT12_RS09015 Protein ID WP_000854814.1
Coordinates 1860179..1860553 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag HZT12_RS09020 Protein ID WP_001285585.1
Coordinates 1860642..1861010 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT12_RS08975 (1855959) 1855959..1857499 + 1541 Protein_1762 IS21-like element ISEc12 family transposase -
HZT12_RS08980 (1857514) 1857514..1858260 + 747 WP_240765510.1 IS21-like element ISEc12 family helper ATPase IstB -
HZT12_RS08985 (1858338) 1858338..1858589 + 252 Protein_1764 hypothetical protein -
HZT12_RS08990 (1858761) 1858761..1859090 + 330 WP_000450409.1 DUF496 family protein -
HZT12_RS08995 (1859191) 1859191..1859325 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
HZT12_RS09000 (1859445) 1859445..1859573 + 129 Protein_1767 transposase domain-containing protein -
HZT12_RS09005 (1859862) 1859862..1859942 - 81 Protein_1768 hypothetical protein -
HZT12_RS09010 (1859988) 1859988..1860182 - 195 WP_000988600.1 DUF5983 family protein -
HZT12_RS09015 (1860179) 1860179..1860553 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
HZT12_RS09020 (1860642) 1860642..1861010 - 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
HZT12_RS09025 (1861084) 1861084..1861305 - 222 WP_000692323.1 DUF987 domain-containing protein -
HZT12_RS09030 (1861368) 1861368..1861634 - 267 Protein_1773 JAB domain-containing protein -
HZT12_RS09040 (1862381) 1862381..1862848 - 468 Protein_1775 PfkB family carbohydrate kinase -
HZT12_RS09045 (1863044) 1863044..1863382 - 339 Protein_1776 transposase -
HZT12_RS09050 (1864270) 1864270..1864434 + 165 WP_001128468.1 hypothetical protein -
HZT12_RS09055 (1864407) 1864407..1864595 + 189 Protein_1778 IS4 family transposase -
HZT12_RS09060 (1864574) 1864574..1864747 + 174 Protein_1779 cobalamin biosynthesis protein -
HZT12_RS09065 (1864899) 1864899..1865444 + 546 WP_000973176.1 bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 1855959..1863160 7201


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T167534 WP_000854814.1 NZ_CP058796:c1860553-1860179 [Shigella flexneri]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T167534 NZ_CP075141:843162-843560 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT167534 WP_001285585.1 NZ_CP058796:c1861010-1860642 [Shigella flexneri]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT167534 NZ_CP075141:842935-843162 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References