Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 887536..888190 Replicon chromosome
Accession NZ_CP058796
Organism Shigella flexneri strain STLIN_8

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HZT12_RS04355 Protein ID WP_000244777.1
Coordinates 887783..888190 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HZT12_RS04350 Protein ID WP_000354046.1
Coordinates 887536..887802 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT12_RS04325 (882705) 882705..883448 + 744 WP_000951964.1 SDR family oxidoreductase -
HZT12_RS04330 (883505) 883505..884938 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
HZT12_RS04335 (884983) 884983..885294 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HZT12_RS04340 (885458) 885458..886117 + 660 WP_000250274.1 hemolysin III family protein -
HZT12_RS04345 (886313) 886313..887293 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
HZT12_RS04350 (887536) 887536..887802 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HZT12_RS04355 (887783) 887783..888190 + 408 WP_000244777.1 protein YgfX Toxin
HZT12_RS04360 (888230) 888230..888751 - 522 WP_001055874.1 flavodoxin FldB -
HZT12_RS04365 (888863) 888863..889759 + 897 WP_000806628.1 site-specific tyrosine recombinase XerD -
HZT12_RS04370 (889784) 889784..890494 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HZT12_RS04375 (890500) 890500..892233 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T167532 WP_000244777.1 NZ_CP058796:887783-888190 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T167532 NZ_CP075141:316762-317130 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT167532 WP_000354046.1 NZ_CP058796:887536-887802 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT167532 NZ_CP075141:316544-316765 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References