Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 93284..93927 Replicon plasmid unnamed1
Accession NZ_CP058791
Organism Shigella flexneri strain STLIN_6

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag HZT08_RS23915 Protein ID WP_001044768.1
Coordinates 93511..93927 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag HZT08_RS23910 Protein ID WP_001261287.1
Coordinates 93284..93514 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT08_RS23905 (89859) 89859..92978 - 3120 WP_000467135.1 hypothetical protein -
HZT08_RS23910 (93284) 93284..93514 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HZT08_RS23915 (93511) 93511..93927 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
HZT08_RS23920 (94089) 94089..96227 - 2139 WP_000350635.1 AAA family ATPase -
HZT08_RS23925 (96581) 96581..96838 + 258 WP_000371887.1 hypothetical protein -
HZT08_RS23930 (96838) 96838..97428 + 591 WP_000194548.1 hypothetical protein -
HZT08_RS23935 (97446) 97446..97793 - 348 WP_000142443.1 DUF6404 family protein -
HZT08_RS23940 (97922) 97922..98257 + 336 WP_057698418.1 colicin E1 family microcin immunity protein -
HZT08_RS23945 (98279) 98279..98764 - 486 Protein_114 colicin Ia central receptor-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - estIa / paa / hlyD / hlyB / hlyA / hlyC 1..137079 137079


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T167527 WP_001044768.1 NZ_CP058791:93511-93927 [Shigella flexneri]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T167527 NZ_CP075140:c4303144-4302860 [Salmonella enterica]
ATGACCTATGAGCTGGAGTTTGATCCACGCGCGCTAAAAGAGTGGCAAAAGCTGGGTGAAACGGTAAAAAAACAGTTCCG
CAAGAAACTTGTCGACGTGTTGTCAAATCCGCGGGTTGAGTCTGCGCGGCTGCGTGAACTGCCAGATTGCTACAAAATCA
AGCTGAAGACCTCAGGCTATCGTTTAGTGTATCAGGTGCAGGACGACGTGGTATTGGTGGTGGTGATTGCTATCGGCAAG
AGAGAGAAATCGGCCGTTTATCGCCAGGCAGACAAACGGCTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT167527 WP_001261287.1 NZ_CP058791:93284-93514 [Shigella flexneri]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT167527 NZ_CP075140:c4303376-4303134 [Salmonella enterica]
ATGGCCACACTTAACGTCCGTTTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATTACCCC
CACAGAAGCTGTCCGGCTGTTGTTTCAATATGTGGCGGAAAACGGGCGTATGCCTGTTAAAACCGTTACGATTAGCGACA
GCGAAGACGCATTGCTACAGACAGTCCGGGAACGACTGGCTAACCCACAGAAAGGAATCAGAGTTAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References