Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 81186..81711 Replicon plasmid unnamed1
Accession NZ_CP058791
Organism Shigella flexneri strain STLIN_6

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag HZT08_RS23860 Protein ID WP_001159868.1
Coordinates 81186..81491 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HZT08_RS23865 Protein ID WP_000813634.1
Coordinates 81493..81711 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT08_RS23840 (77566) 77566..78006 - 441 Protein_93 RepB family plasmid replication initiator protein -
HZT08_RS23845 (78098) 78098..79326 + 1229 WP_088895425.1 IS3-like element IS2 family transposase -
HZT08_RS23850 (79331) 79331..79657 - 327 Protein_95 RepB family plasmid replication initiator protein -
HZT08_RS23855 (80379) 80379..81185 - 807 WP_000016982.1 site-specific integrase -
HZT08_RS23860 (81186) 81186..81491 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
HZT08_RS23865 (81493) 81493..81711 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HZT08_RS23870 (82419) 82419..83414 + 996 WP_000246635.1 hypothetical protein -
HZT08_RS23875 (83418) 83418..84350 + 933 WP_000991831.1 S-4TM family putative pore-forming effector -
HZT08_RS23880 (84648) 84648..85736 + 1089 WP_000952231.1 hypothetical protein -
HZT08_RS23885 (85738) 85738..85893 + 156 WP_240766700.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - estIa / paa / hlyD / hlyB / hlyA / hlyC 1..137079 137079
- flank IS/Tn - - 85963..87171 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T167526 WP_001159868.1 NZ_CP058791:c81491-81186 [Shigella flexneri]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T167526 NZ_CP075140:c4163099-4162812 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAACAAACACGGAATCCGCTTTGAGGATGCGGT
GCTGGTGTTTGACGATCCCCGACACTTGTCGCGTCAGGAACGTTACGAGAATGGAGAATACCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTTATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTATGAAGTTATCCGTATCATCAGC
ACCCGCAAGGCGGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT167526 WP_000813634.1 NZ_CP058791:c81711-81493 [Shigella flexneri]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT167526 NZ_CP075140:c4162825-4162523 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAATGTACCTGCGCTTAGTGCTCAGCATGAGGCTGAACTCAAGGCGCTGGCGAA
AAAGTCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGAATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACCCAAGCCTCCGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACACGGCTCAATGCTATTCTGCGCGAAGCGATGCTGCGAGATCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References