Detailed information of TA system TA167522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 45241..45866
• Replicon: plasmid unnamed1
• Accession: NZ_CP058791
• Organism: Shigella flexneri strain STLIN_6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HZT08_RS23635
• Protein ID: WP_000911314.1
• Coordinates: 45468..45866 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: HZT08_RS23630
• Protein ID: WP_000450532.1
• Coordinates: 45241..45468 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT08_RS23630 (45241)	45241..45468	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HZT08_RS23635 (45468)	45468..45866	+	399	WP_000911314.1	type II toxin-antitoxin system VapC family toxin	Toxin
HZT08_RS23640 (45875)	45875..48028	-	2154	WP_240766716.1	type IV conjugative transfer system coupling protein TraD	-
HZT08_RS23645 (48281)	48281..49012	-	732	WP_000850426.1	conjugal transfer complement resistance protein TraT	-
HZT08_RS23650 (49026)	49026..49535	-	510	WP_000628103.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	estIa / paa / hlyD / hlyB / hlyA / hlyC	1..137079	137079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14814.12 Da
• Isoelectric Point: 7.8604

>T167522 WP_000911314.1 NZ_CP058791:45468-45866 [Shigella flexneri]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T167522 NZ_CP075140:858242-858655 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGAATTACGCGTCTCATGGCGCGCTCAGTGGATCTCGTTGCTTATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCATTGTGGATGATGCTACTGTCGCTGGTCGTGTTTGACTGCG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAAATCAAGCTGCTAATGGATGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTTTTGCGTCCGCCCTGGTTACTGAGGAGCGGGATGGTGTTGCGTTTACGCGCTGAGTCTGGGCACCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGCAGCGGAATGGCGTGAGTTGCGTCGGATAATGTTACAGCAACCAATAT
CGCGCCAACACTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT167522 WP_000450532.1 NZ_CP058791:45241-45468 [Shigella flexneri]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT167522 NZ_CP075140:857995-858261 [Salmonella enterica]
ATGGACATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGCGACGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTGATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5