Detailed information of TA system TA167512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3537307..3538144
• Replicon: chromosome
• Accession: NZ_CP058790
• Organism: Shigella flexneri strain STLIN_6

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: HZT08_RS17235
• Protein ID: WP_000227784.1
• Coordinates: 3537602..3538144 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: HZT08_RS17230
• Protein ID: WP_001297137.1
• Coordinates: 3537307..3537618 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT08_RS17205 (3532327)	3532327..3533274	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
HZT08_RS17210 (3533296)	3533296..3535287	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
HZT08_RS17215 (3535277)	3535277..3535891	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
HZT08_RS17220 (3535891)	3535891..3536220	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
HZT08_RS17225 (3536232)	3536232..3537122	+	891	WP_000971336.1	heme o synthase	-
HZT08_RS17230 (3537307)	3537307..3537618	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
HZT08_RS17235 (3537602)	3537602..3538144	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
HZT08_RS17240 (3538200)	3538200..3539135	-	936	WP_001368479.1	tetratricopeptide repeat protein	-
HZT08_RS17245 (3539543)	3539543..3540907	+	1365	WP_001000960.1	MFS transporter	-
HZT08_RS17250 (3541035)	3541035..3541526	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
HZT08_RS17255 (3541694)	3541694..3542605	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T167512 WP_000227784.1 NZ_CP058790:3537602-3538144 [Shigella flexneri]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T167512 NZ_CP075138:c4129841-4129557 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT167512 WP_001297137.1 NZ_CP058790:3537307-3537618 [Shigella flexneri]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT167512 NZ_CP075138:c4130073-4129831 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3