Detailed information of TA system TA167509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2458073..2458711
• Replicon: chromosome
• Accession: NZ_CP058790
• Organism: Shigella flexneri strain STLIN_6

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HZT08_RS11900
• Protein ID: WP_000813794.1
• Coordinates: 2458535..2458711 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HZT08_RS11895
• Protein ID: WP_001270285.1
• Coordinates: 2458073..2458489 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT08_RS11875 (2453225)	2453225..2454166	-	942	WP_001251315.1	ABC transporter permease	-
HZT08_RS11880 (2454167)	2454167..2455180	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
HZT08_RS11885 (2455198)	2455198..2456343	-	1146	WP_240766269.1	ABC transporter substrate-binding protein	-
HZT08_RS11890 (2456588)	2456588..2457994	-	1407	WP_000760594.1	PLP-dependent aminotransferase family protein	-
HZT08_RS11895 (2458073)	2458073..2458489	-	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HZT08_RS11900 (2458535)	2458535..2458711	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HZT08_RS11905 (2458933)	2458933..2459163	+	231	WP_000494244.1	YncJ family protein	-
HZT08_RS11910 (2459255)	2459255..2461216	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HZT08_RS11915 (2461289)	2461289..2461825	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HZT08_RS11920 (2461917)	2461917..2463092	+	1176	WP_001236268.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2463132..2464280	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T167509 WP_000813794.1 NZ_CP058790:c2458711-2458535 [Shigella flexneri]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T167509 NZ_CP075138:c1894817-1894488 [Salmonella enterica]
ATGAACTACACTATTGAGTACTACAACGAGGAAGTCCGGATTGAGGTGGATCAGCTTCCATTGAGTATGCGAGCCAGATA
TCAGCACCTTGTTGAACGAATGAAGGTGTATGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGATGGGCTTT
TTGAGCTTCGGATTAAAGGCAGCGATGGCATCGCTCGCGTTTTTTACTGCACCCTGGCAGGGAAGCGCATCGTCATGCTG
CACAGCTTTATAAAGAAGACTCAGAAAACCCCTGTAGCTGAACGCAAAAAGGCAGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT167509 WP_001270285.1 NZ_CP058790:c2458489-2458073 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT167509 NZ_CP075138:c1894498-1894190 [Salmonella enterica]
ATGACTGGTAAACGTAATGCGCCAACTATAACGCACGATGAAATGGCCGCTAAATGGATGGAAGACCCAGCGTTCAAAGC
TGAATATGACGCCATTGCTGATGAGTTCGCGTTACTTGACGAAATGCTGGCCGCACGTAAAGAAGCAGGTCTCACCCAGG
CTGAAGTTGCTGAACGGATGGGAACAAAAGCCACTGCTATAACCAGAATGGAAAGCAACCTTGCTGCTGGCGTCAGCGGA
CCATCTTTTACTACGCTGAAAAAGTTCGCACAGGCAACCGGGAAAAAACTCCAGATCCGCTTCGTGTAA

Structures

Toxin

• PDB: 6HPB