Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1334301..1334926 Replicon chromosome
Accession NZ_CP058790
Organism Shigella flexneri strain STLIN_6

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag HZT08_RS06470 Protein ID WP_000911329.1
Coordinates 1334528..1334926 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HZT08_RS06465 Protein ID WP_000450524.1
Coordinates 1334301..1334528 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT08_RS06440 (1330103) 1330103..1330573 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
HZT08_RS06445 (1330573) 1330573..1331145 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
HZT08_RS06450 (1331291) 1331291..1332169 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
HZT08_RS06455 (1332186) 1332186..1333220 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
HZT08_RS06460 (1333433) 1333433..1334146 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HZT08_RS06465 (1334301) 1334301..1334528 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HZT08_RS06470 (1334528) 1334528..1334926 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
HZT08_RS06475 (1335073) 1335073..1335936 + 864 WP_001267498.1 neutral zinc metallopeptidase -
HZT08_RS06480 (1335951) 1335951..1337966 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
HZT08_RS06485 (1338040) 1338040..1338738 + 699 WP_000679823.1 esterase -
HZT08_RS06490 (1338848) 1338848..1339048 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T167498 WP_000911329.1 NZ_CP058790:1334528-1334926 [Shigella flexneri]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T167498 NZ_CP075137:3529305-3529523 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT167498 WP_000450524.1 NZ_CP058790:1334301-1334528 [Shigella flexneri]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT167498 NZ_CP075137:3528903-3529277 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References