Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1005515..1006098 Replicon chromosome
Accession NZ_CP058790
Organism Shigella flexneri strain STLIN_6

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag HZT08_RS04880 Protein ID WP_000254745.1
Coordinates 1005763..1006098 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag HZT08_RS04875 Protein ID WP_000581937.1
Coordinates 1005515..1005763 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT08_RS04865 (1001854) 1001854..1003155 + 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
HZT08_RS04870 (1003203) 1003203..1005437 + 2235 WP_000226815.1 GTP pyrophosphokinase -
HZT08_RS04875 (1005515) 1005515..1005763 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HZT08_RS04880 (1005763) 1005763..1006098 + 336 WP_000254745.1 endoribonuclease MazF Toxin
HZT08_RS04885 (1006169) 1006169..1006960 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HZT08_RS04890 (1007188) 1007188..1008825 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HZT08_RS04895 (1008913) 1008913..1010211 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T167497 WP_000254745.1 NZ_CP058790:1005763-1006098 [Shigella flexneri]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T167497 NZ_CP075137:c2054318-2054007 [Salmonella enterica]
ATGCATGTCATATCAAAAGAGCCTTTTGATGAAGCGGCAAGACGATATCCCAATGACTCGTTAGCCATAAAAGCCCTGTA
TCGCTTAGTCCGCGAAAAAGATTTCTCTTCTCCGGCAGAGCTACGAAAGGTCATACCGAGTCTCGATAACTTTAAGTACC
GTAACAAGTGGTGGGTACTGGATGTCGGAGGCAACAACCTGAGGGTGATCGCTTACATCAACTTTATAAACAAACGCTTT
TATGTGAAACACATCGCCACTCACGCTGAATATGACAAGCTGACCCGCTACTACAGGGAGAATAAAGAATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT167497 WP_000581937.1 NZ_CP058790:1005515-1005763 [Shigella flexneri]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT167497 NZ_CP075137:c2054010-2053591 [Salmonella enterica]
ATGATTGCCAACACCGCAAAAGCTATCGAAGCCACAAAGGCGCTGGTCGCCGCCGTTCCTTTCCTGGGTGGCAGCGCGTC
AGAGAAAGATTATCGTGATGCGCTGGCGCTGGTTGATTATCTTATCGAAAATGACGATGAAAACCCGCTGATTGACTTCC
TGGCAAGCAAAATTGCCGAGTACGAAGATAACAGCAAGCAGTTCGCCGAGTTCAACAAATCTGTTGCTGAAATGCCGGTT
GGCGTTGCCCTGCTGCGCACGCTAATTGACCAGTACAAACTCTCATACTCAGATCTTAAAGAAGAAATCGGTTCCAAATC
ACTGGTAAGTCAGATCCTGTCCGGACAGCGCTCGTTAACGATTACGCACATCAAAGCGCTTTCAGCACGATTTGGCGTGA
AACCTGAGTGGTTCTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References