Detailed information of TA system TA167494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 275795..276426
• Replicon: chromosome
• Accession: NZ_CP058790
• Organism: Shigella flexneri strain STLIN_6

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9Y2A7
• Locus tag: HZT08_RS01270
• Protein ID: WP_001259385.1
• Coordinates: 275795..276070 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: HZT08_RS01275
• Protein ID: WP_000593555.1
• Coordinates: 276067..276426 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT08_RS01255 (270799)	270799..271866	+	1068	WP_000361467.1	HlyD family secretion protein	-
HZT08_RS01260 (271863)	271863..274598	+	2736	WP_000149165.1	ribosome-associated ATPase/putative transporter RbbA	-
HZT08_RS01265 (274598)	274598..275722	+	1125	WP_001314210.1	ABC transporter permease	-
HZT08_RS01270 (275795)	275795..276070	+	276	WP_001259385.1	type II toxin-antitoxin system HicA family toxin	Toxin
HZT08_RS01275 (276067)	276067..276426	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HZT08_RS01280 (276546)	276546..276947	-	402	WP_240766475.1	nickel-responsive transcriptional regulator NikR	-
HZT08_RS01285 (276953)	276953..277759	-	807	WP_000173666.1	nickel import ATP-binding protein NikE	-
HZT08_RS01290 (277756)	277756..278520	-	765	WP_240766476.1	nickel import ATP-binding protein NikD	-
HZT08_RS01295 (278520)	278520..279353	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
HZT08_RS01300 (279350)	279350..280294	-	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	266063..276426	10363

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10199.94 Da
• Isoelectric Point: 10.7076

>T167494 WP_001259385.1 NZ_CP058790:275795-276070 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T167494 NZ_CP075137:c1017616-1017089 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAATCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGATAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTTTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTATTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT167494 WP_000593555.1 NZ_CP058790:276067-276426 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT167494 NZ_CP075137:c1017903-1017613 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A4P7TTW8

Antitoxin

• AlphaFold DB: A0A7U9LKZ6