Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 68392..68993 Replicon plasmid unnamed1
Accession NZ_CP058787
Organism Shigella flexneri strain STLIN_4

Toxin (Protein)


Gene name doc Uniprot ID A0A5M4JUN5
Locus tag HZT07_RS23440 Protein ID WP_001216041.1
Coordinates 68613..68993 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag HZT07_RS23435 Protein ID WP_001190712.1
Coordinates 68392..68613 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT07_RS23370 (HZT07_23210) 63586..63780 + 195 WP_000224731.1 hypothetical protein -
HZT07_RS23375 (HZT07_23215) 63782..63991 + 210 WP_240763459.1 hypothetical protein -
HZT07_RS23380 63988..64113 + 126 Protein_82 DUF551 domain-containing protein -
HZT07_RS23385 64570..64755 + 186 WP_235392042.1 DUF551 domain-containing protein -
HZT07_RS23390 (HZT07_23225) 64752..65012 + 261 WP_063074809.1 hypothetical protein -
HZT07_RS23395 (HZT07_23235) 65298..65714 + 417 WP_063074823.1 hypothetical protein -
HZT07_RS23400 (HZT07_23240) 65727..66020 + 294 WP_075660654.1 hypothetical protein -
HZT07_RS23405 (HZT07_23245) 66020..66196 + 177 WP_157902266.1 hypothetical protein -
HZT07_RS23410 (HZT07_23250) 66276..66485 + 210 WP_075660655.1 hypothetical protein -
HZT07_RS23415 (HZT07_23255) 66482..67240 + 759 WP_159195145.1 hypothetical protein -
HZT07_RS23420 (HZT07_23260) 67286..67552 + 267 WP_000903204.1 hypothetical protein -
HZT07_RS23425 (HZT07_23265) 67555..67806 + 252 WP_063074807.1 DNA polymerase III subunit theta -
HZT07_RS23430 (HZT07_23270) 67930..68319 + 390 WP_168420616.1 S24 family peptidase -
HZT07_RS23435 (HZT07_23275) 68392..68613 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HZT07_RS23440 (HZT07_23280) 68613..68993 + 381 WP_001216041.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HZT07_RS23445 (HZT07_23285) 69077..69877 - 801 WP_000432092.1 hypothetical protein -
HZT07_RS23450 (HZT07_23290) 69884..70546 - 663 WP_240763460.1 DUF2829 domain-containing protein -
HZT07_RS23455 (HZT07_23295) 70561..71055 - 495 WP_000640903.1 dUTP diphosphatase -
HZT07_RS23460 (HZT07_23300) 71052..71528 - 477 WP_000861173.1 hypothetical protein -
HZT07_RS23465 (HZT07_23305) 71525..71869 - 345 WP_001557739.1 hypothetical protein -
HZT07_RS23470 (HZT07_23310) 71943..72971 - 1029 WP_001292231.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..92052 92052


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13635.52 Da        Isoelectric Point: 5.6406

>T167490 WP_001216041.1 NZ_CP058787:68613-68993 [Shigella flexneri]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Download         Length: 381 bp

>T167490 NZ_CP075137:c87088-86720 [Salmonella enterica]
ATGCAAAACGTTTTATCACTGCCTTCACCGGCAGCGGCCTCGTCACGCCTGTCGCCCGTCGAAGCCTGGCAAAAACTGCT
GTCGTACCTGCTGCAACGCCACTACGGCCTTGAACTTAACGACACGCCATTCTGCGACGATATTGTTATTCAGGAACATA
TCAACGCAGGCGTTGCGCTTGCCGATGCCGTTAACTTTCTGGTGGAAAAGTACGGACTTGTTCGTATCGATCGCAAGGGA
TTTAGCTGGCAGGAACAATCACCCTATCTCCGGGCTGTCGATATTCTGCGGGCGCGGAAAGCAACGGGGTTGTTAACACG
TTGCAAAAAACACAATGGTAATCAGCCTGATGGCAGATTCACTCTATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT167490 WP_001190712.1 NZ_CP058787:68392-68613 [Shigella flexneri]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT167490 NZ_CP075137:c87432-87103 [Salmonella enterica]
ATGCCCCAAAACCAACCCTCCGCGCCTGTCTGGGGCCTGCGGAGCGATATCACGCCCTCGTTTGGCGCTCGGTTAGTGCA
GGAAGGTTGTCGCCTGCATTTTCTGCCCGACCGCGCCAGCCTTCGCGGTGATTTCACTGCGGAACAACTACACTCGCTGG
ATATCACCTTTCCGCAGTTTGTGAAGCAACTGGAATCGGCGCTGAAAAGCGGGGCGCTCGATCCGCACCAGTCGCGCCGC
TACAGCACCATCCTCAATGGCCTGACCTGCGAAGCCGACACCAACGGTAGTCACGGCTACGTTTACCTCACTATCTACCC
AGCCGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5M4JUN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References