167487

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4005465..4006041 Replicon chromosome
Accession NZ_CP058786
Organism Shigella flexneri strain STLIN_4

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HZT07_RS19585 Protein ID WP_001297643.1
Coordinates 4005754..4006041 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HZT07_RS19580 Protein ID WP_000063156.1
Coordinates 4005465..4005767 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT07_RS19565 (4002061) 4002061..4004211 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
HZT07_RS19570 (4004261) 4004261..4004464 + 204 WP_000467859.1 YbdD/YjiX family protein -
HZT07_RS19575 (4004475) 4004475..4005431 + 957 WP_001297640.1 GTPase -
HZT07_RS19580 (4005465) 4005465..4005767 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HZT07_RS19585 (4005754) 4005754..4006041 - 288 WP_001297643.1 BrnT family toxin Toxin
HZT07_RS19590 (4006240) 4006240..4006937 - 698 Protein_3845 IS1 family transposase -
HZT07_RS19595 (4006994) 4006994..4007089 + 96 Protein_3846 dienelactone hydrolase family protein -
HZT07_RS19600 (4007235) 4007235..4008467 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
HZT07_RS19605 (4008508) 4008508..4009788 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4006240..4006449 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T167487 WP_001297643.1 NZ_CP058786:c4006041-4005754 [Shigella flexneri]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T167487 NZ_CP075135:c4679248-4678937 [Salmonella enterica]
ATGCAATTCATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCATCGTAGTCAACGGTATGAGGTTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT167487 WP_000063156.1 NZ_CP058786:c4005767-4005465 [Shigella flexneri]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT167487 NZ_CP075135:c4678936-4678646 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References