Detailed information of TA system TA167472

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1865478..1866313
• Replicon: chromosome
• Accession: NZ_CP058786
• Organism: Shigella flexneri strain STLIN_4

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X9TM58
• Locus tag: HZT07_RS09030
• Protein ID: WP_000854761.1
• Coordinates: 1865478..1865855 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: HZT07_RS09035
• Protein ID: WP_001295723.1
• Coordinates: 1865945..1866313 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT07_RS08990 (1860744)	1860744..1861802	+	1059	WP_001200892.1	FUSC family protein	-
HZT07_RS08995 (1861974)	1861974..1862303	+	330	WP_000450409.1	DUF496 family protein	-
HZT07_RS09000 (1862404)	1862404..1862769	-	366	WP_001282181.1	EutP/PduV family microcompartment system protein	-
HZT07_RS09005 (1863040)	1863040..1863429	-	390	WP_001445118.1	IS110 family transposase	-
HZT07_RS09010 (1863618)	1863618..1863842	-	225	Protein_1767	transposase	-
HZT07_RS09015 (1864020)	1864020..1864722	+	703	Protein_1768	IS66-like element ISEc22 family transposase	-
HZT07_RS09020 (1865153)	1865153..1865233	-	81	Protein_1769	hypothetical protein	-
HZT07_RS09025 (1865333)	1865333..1865481	-	149	Protein_1770	DUF5983 family protein	-
HZT07_RS09030 (1865478)	1865478..1865855	-	378	WP_000854761.1	TA system toxin CbtA family protein	Toxin
HZT07_RS09035 (1865945)	1865945..1866313	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HZT07_RS09040 (1866476)	1866476..1866697	-	222	WP_000692345.1	DUF987 domain-containing protein	-
HZT07_RS09045 (1866999)	1866999..1867568	+	570	WP_000221554.1	inovirus Gp2 family protein	-
HZT07_RS09050 (1867734)	1867734..1868126	+	393	WP_001352248.1	putative zinc ribbon protein	-
HZT07_RS09055 (1868136)	1868136..1869104	-	969	Protein_1776	IS110 family transposase	-
HZT07_RS09060 (1869289)	1869289..1869693	+	405	WP_001221631.1	zinc-ribbon domain-containing protein	-
HZT07_RS09065 (1869984)	1869984..1870646	+	663	Protein_1778	ISL3 family transposase	-
HZT07_RS09070 (1870710)	1870710..1870970	+	261	Protein_1779	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1864032..1864718	686

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14205.25 Da
• Isoelectric Point: 7.3249

>T167472 WP_000854761.1 NZ_CP058786:c1865855-1865478 [Shigella flexneri]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T167472 NZ_CP075134:2015551-2015654 [Salmonella enterica subsp. enterica serovar Saintpaul]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT167472 WP_001295723.1 NZ_CP058786:c1866313-1865945 [Shigella flexneri]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT167472 NZ_CP075134:c2015667-2015513 [Salmonella enterica subsp. enterica serovar Saintpaul]
TTTTAATCTAAATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGC
TAAAAGTTGGCATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Structures

Toxin

• AlphaFold DB: A0A1X9TM58

Antitoxin

• AlphaFold DB: S1NM52