Detailed information of TA system TA167470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1027720..1028303
• Replicon: chromosome
• Accession: NZ_CP058786
• Organism: Shigella flexneri strain STLIN_4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HZT07_RS05040
• Protein ID: WP_049253269.1
• Coordinates: 1027968..1028303 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1V2T5M9
• Locus tag: HZT07_RS05035
• Protein ID: WP_000581941.1
• Coordinates: 1027720..1027968 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT07_RS05025 (1024059)	1024059..1025360	+	1302	WP_023281448.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
HZT07_RS05030 (1025408)	1025408..1027642	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
HZT07_RS05035 (1027720)	1027720..1027968	+	249	WP_000581941.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HZT07_RS05040 (1027968)	1027968..1028303	+	336	WP_049253269.1	endoribonuclease MazF	Toxin
HZT07_RS05045 (1028374)	1028374..1029165	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
HZT07_RS05050 (1029393)	1029393..1031030	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
HZT07_RS05055 (1031118)	1031118..1032416	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12197.18 Da
• Isoelectric Point: 8.7218

>T167470 WP_049253269.1 NZ_CP058786:1027968-1028303 [Shigella flexneri]
MVSRYVPDMGDLIWVDFDPTKGSEQARHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T167470 NZ_CP075134:857269-857667 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTTGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9385.76 Da
• Isoelectric Point: 4.4665

>AT167470 WP_000581941.1 NZ_CP058786:1027720-1027968 [Shigella flexneri]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT167470 NZ_CP075134:857042-857269 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGATGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1V2T5M9