Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 30545..31188 Replicon plasmid unnamed1
Accession NZ_CP058781
Organism Shigella flexneri strain STLIN_17

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag HZT03_RS22720 Protein ID WP_001044768.1
Coordinates 30545..30961 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag HZT03_RS22725 Protein ID WP_001261287.1
Coordinates 30958..31188 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT03_RS22705 (HZT03_22545) 27044..27634 - 591 WP_000194575.1 hypothetical protein -
HZT03_RS22710 (HZT03_22550) 27634..27891 - 258 WP_000343085.1 hypothetical protein -
HZT03_RS22715 (HZT03_22555) 28245..30383 + 2139 WP_000350638.1 AAA family ATPase -
HZT03_RS22720 (HZT03_22560) 30545..30961 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
HZT03_RS22725 (HZT03_22565) 30958..31188 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HZT03_RS22730 (HZT03_22570) 31484..31756 + 273 WP_061602529.1 hypothetical protein -
HZT03_RS22735 (HZT03_22575) 31803..32507 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
HZT03_RS22740 (HZT03_22580) 32554..32988 - 435 WP_113403791.1 Hg(II)-responsive transcriptional regulator -
HZT03_RS22745 (HZT03_22585) 33060..33410 + 351 WP_001294663.1 mercuric transport protein MerT -
HZT03_RS22750 (HZT03_22590) 33424..33699 + 276 WP_000732292.1 mercury resistance system periplasmic binding protein MerP -
HZT03_RS22755 (HZT03_22595) 33735..34157 + 423 WP_001340589.1 organomercurial transporter MerC -
HZT03_RS22760 (HZT03_22600) 34209..35903 + 1695 WP_000105636.1 mercury(II) reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid oqxB / oqxA / aph(3')-Ia / floR / tet(A) / blaTEM-1A - 1..99490 99490
- flank IS/Tn - - 31803..32507 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T167464 WP_001044768.1 NZ_CP058781:c30961-30545 [Shigella flexneri]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T167464 NZ_CP075132:c4477087-4476596 [Salmonella enterica subsp. enterica serovar Inverness]
ATGATCTCCGCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAGCGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAAGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCATGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT167464 WP_001261287.1 NZ_CP058781:c31188-30958 [Shigella flexneri]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT167464 NZ_CP075132:c4477377-4477084 [Salmonella enterica subsp. enterica serovar Inverness]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCCGCCCGCGCTGCCGCAGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCACAGGCTTATCAGGAATTTCTGGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References