Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3658950..3659737 Replicon chromosome
Accession NZ_CP058780
Organism Shigella flexneri strain STLIN_17

Toxin (Protein)


Gene name yeeV Uniprot ID A0A377E3D6
Locus tag HZT03_RS17865 Protein ID WP_001194695.1
Coordinates 3659360..3659737 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2H9G3E7
Locus tag HZT03_RS17860 Protein ID WP_000066236.1
Coordinates 3658950..3659309 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT03_RS17815 (3654190) 3654190..3654642 + 453 WP_001020418.1 hypothetical protein -
HZT03_RS17820 (3654639) 3654639..3655091 + 453 WP_000734138.1 hypothetical protein -
HZT03_RS17825 (3655154) 3655154..3655696 + 543 WP_001104018.1 DUF4339 domain-containing protein -
HZT03_RS17830 (3655755) 3655755..3656207 + 453 WP_001061894.1 IrmA family protein -
HZT03_RS17835 (3656284) 3656284..3656517 + 234 WP_001114682.1 DUF905 domain-containing protein -
HZT03_RS17840 (3656637) 3656637..3657455 + 819 WP_152961050.1 DUF932 domain-containing protein -
HZT03_RS17845 (3657723) 3657723..3658193 + 471 WP_000131762.1 antirestriction protein -
HZT03_RS17850 (3658205) 3658205..3658684 + 480 WP_000437750.1 DNA repair protein RadC -
HZT03_RS17855 (3658705) 3658705..3658926 + 222 WP_000691981.1 DUF987 domain-containing protein -
HZT03_RS17860 (3658950) 3658950..3659309 + 360 WP_000066236.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HZT03_RS17865 (3659360) 3659360..3659737 + 378 WP_001194695.1 TA system toxin CbtA family protein Toxin
HZT03_RS17870 (3659734) 3659734..3660225 + 492 WP_000777682.1 DUF5983 family protein -
HZT03_RS17875 (3660257) 3660257..3660460 + 204 WP_000413747.1 DUF957 domain-containing protein -
HZT03_RS17880 (3660541) 3660541..3661385 + 845 Protein_3514 DUF4942 domain-containing protein -
HZT03_RS17890 (3661727) 3661727..3662458 - 732 WP_001300756.1 DNA polymerase III subunit epsilon -
HZT03_RS17895 (3662523) 3662523..3662990 + 468 WP_000917883.1 ribonuclease HI -
HZT03_RS17900 (3662987) 3662987..3663709 - 723 WP_001297210.1 class I SAM-dependent methyltransferase -
HZT03_RS17905 (3663743) 3663743..3664498 + 756 WP_001052717.1 hydroxyacylglutathione hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3647211..3669408 22197


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(15-114)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14180.97 Da        Isoelectric Point: 7.4209

>T167462 WP_001194695.1 NZ_CP058780:3659360-3659737 [Shigella flexneri]
MQTQPLSSTQEATSRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKATGLMTRNDYRTVTDITTGKYRGGHR

Download         Length: 378 bp

>T167462 NZ_CP075132:3656382-3656600 [Salmonella enterica subsp. enterica serovar Inverness]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACAATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13127.02 Da        Isoelectric Point: 7.0261

>AT167462 WP_000066236.1 NZ_CP058780:3658950-3659309 [Shigella flexneri]
MSNKIPTVNHDITEPWWGLKRSITPCFGARLVQSGNCLHYLADRASITGQFSDGDLRHLDQAFPLLLKQLELMLTSGELT
PRHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Download         Length: 360 bp

>AT167462 NZ_CP075132:3655980-3656354 [Salmonella enterica subsp. enterica serovar Inverness]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAAC
ATATTGCAACTTTCGCTCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377E3D6

Antitoxin

Source ID Structure
AlphaFold DB A0A2H9G3E7

References