Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 867426..868080 Replicon chromosome
Accession NZ_CP058780
Organism Shigella flexneri strain STLIN_17

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HZT03_RS04300 Protein ID WP_000244777.1
Coordinates 867673..868080 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HZT03_RS04295 Protein ID WP_000354046.1
Coordinates 867426..867692 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT03_RS04270 (862595) 862595..863338 + 744 WP_000951948.1 SDR family oxidoreductase -
HZT03_RS04275 (863395) 863395..864828 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
HZT03_RS04280 (864873) 864873..865184 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HZT03_RS04285 (865348) 865348..866007 + 660 WP_000250274.1 hemolysin III family protein -
HZT03_RS04290 (866203) 866203..867183 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HZT03_RS04295 (867426) 867426..867692 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HZT03_RS04300 (867673) 867673..868080 + 408 WP_000244777.1 protein YgfX Toxin
HZT03_RS04305 (868120) 868120..868641 - 522 WP_001055874.1 flavodoxin FldB -
HZT03_RS04310 (868753) 868753..869649 + 897 WP_000806639.1 site-specific tyrosine recombinase XerD -
HZT03_RS04315 (869674) 869674..870384 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HZT03_RS04320 (870390) 870390..872123 + 1734 WP_094337022.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T167446 WP_000244777.1 NZ_CP058780:867673-868080 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T167446 NZ_CP075129:2057478-2057581 [Salmonella enterica subsp. enterica serovar Rubislaw]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT167446 WP_000354046.1 NZ_CP058780:867426-867692 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT167446 NZ_CP075129:c2057583-2057440 [Salmonella enterica subsp. enterica serovar Rubislaw]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References