Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 82250..82775 Replicon plasmid unnamed1
Accession NZ_CP058772
Organism Shigella flexneri strain STLIN_11

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag HZS97_RS24085 Protein ID WP_013023785.1
Coordinates 82470..82775 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID -
Locus tag HZS97_RS24080 Protein ID WP_284527033.1
Coordinates 82250..82468 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS97_RS24055 (77462) 77462..78148 + 687 Protein_85 IS1 family transposase -
HZS97_RS24060 (78400) 78400..79423 - 1024 Protein_86 DNA helicase UvrD -
HZS97_RS24065 (79408) 79408..80973 - 1566 WP_151316214.1 AAA family ATPase -
HZS97_RS24070 (81048) 81048..81463 - 416 Protein_88 type II toxin-antitoxin system VapC family toxin -
HZS97_RS24075 (81460) 81460..81690 - 231 WP_284527032.1 type II toxin-antitoxin system VapB family antitoxin -
HZS97_RS24080 (82250) 82250..82468 + 219 WP_284527033.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HZS97_RS24085 (82470) 82470..82775 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
HZS97_RS24090 (82776) 82776..83582 + 807 WP_284527034.1 site-specific integrase -
HZS97_RS24095 (84304) 84304..85060 + 757 Protein_93 replication initiation protein RepE -
HZS97_RS24100 (85648) 85648..86815 + 1168 Protein_94 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / aac(3)-IId / aph(3')-Ia / sul3 / lnu(F) / ant(3'')-Ia / tet(A) / sul2 / floR / dfrA12 / aadA2 / cmlA1 / tet(M) / sitABCD iucA / iucB / iucB / iucC / iucC / iucD / iutA / iutA / iutA 1..121463 121463
- flank IS/Tn - - 77801..78148 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T167436 WP_013023785.1 NZ_CP058772:82470-82775 [Shigella flexneri]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T167436 NZ_CP075128:c2849186-2849083 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8370.27 Da        Isoelectric Point: 4.5203

>AT167436 WP_284527033.1 NZ_CP058772:82250-82468 [Shigella flexneri]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT167436 NZ_CP075128:2849074-2849224 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
TTTAAAAACAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAA
AGTTGGCATTAATGCAGGCTAAGTCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure

References