Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4651082..4651698 Replicon chromosome
Accession NZ_CP058771
Organism Shigella flexneri strain STLIN_11

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag HZS97_RS22570 Protein ID WP_284526834.1
Coordinates 4651082..4651456 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID S1E7W9
Locus tag HZS97_RS22575 Protein ID WP_001086388.1
Coordinates 4651456..4651698 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS97_RS22555 (4648585) 4648585..4649487 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
HZS97_RS22560 (4649484) 4649484..4650119 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
HZS97_RS22565 (4650116) 4650116..4651045 + 930 WP_000027703.1 formate dehydrogenase accessory protein FdhE -
HZS97_RS22570 (4651082) 4651082..4651456 - 375 WP_284526834.1 type II toxin-antitoxin system VapC family toxin Toxin
HZS97_RS22575 (4651456) 4651456..4651698 - 243 WP_001086388.1 protein YiiF Antitoxin
HZS97_RS22580 (4651916) 4651916..4652134 - 219 WP_001295676.1 CopG family transcriptional regulator -
HZS97_RS22585 (4653738) 4653738..4654625 - 888 Protein_4416 hypothetical protein -
HZS97_RS22590 (4655036) 4655036..4655977 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
HZS97_RS22595 (4656022) 4656022..4656459 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13946.17 Da        Isoelectric Point: 7.0126

>T167435 WP_284526834.1 NZ_CP058771:c4651456-4651082 [Shigella flexneri]
MGKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T167435 NZ_CP075128:831648-832061 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
GTGGTCCTGTGGCAATCTGAATTACGCGTCTCATGGCGCGCTCAGTGGATCTCGTTGCTTATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCATTGTGGATGATGCTACTGTCGCTGGTCGTGTTTGACTGCG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAAATCAAGCTGCTAATGGATGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTTTTGCGTCCGCCCTGGTTACTGAGGAGCGGGATGGTGTTGCGTTTACGCGCTGAGTCTGGGCACCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGCAGCGGAATGGCGTGAGTTGCGTCGGATAATGTTACAGCAACCAATAT
CGCGCCAACACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9244.40 Da        Isoelectric Point: 4.2092

>AT167435 WP_001086388.1 NZ_CP058771:c4651698-4651456 [Shigella flexneri]
MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVDQYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT167435 NZ_CP075128:831401-831667 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
ATGGACATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGCGACGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTGATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2K5J
AlphaFold DB A0A4P7TTH6

References