Detailed information of TA system TA167435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4651082..4651698
• Replicon: chromosome
• Accession: NZ_CP058771
• Organism: Shigella flexneri strain STLIN_11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HZS97_RS22570
• Protein ID: WP_284526834.1
• Coordinates: 4651082..4651456 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: S1E7W9
• Locus tag: HZS97_RS22575
• Protein ID: WP_001086388.1
• Coordinates: 4651456..4651698 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS97_RS22555 (4648585)	4648585..4649487	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HZS97_RS22560 (4649484)	4649484..4650119	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HZS97_RS22565 (4650116)	4650116..4651045	+	930	WP_000027703.1	formate dehydrogenase accessory protein FdhE	-
HZS97_RS22570 (4651082)	4651082..4651456	-	375	WP_284526834.1	type II toxin-antitoxin system VapC family toxin	Toxin
HZS97_RS22575 (4651456)	4651456..4651698	-	243	WP_001086388.1	protein YiiF	Antitoxin
HZS97_RS22580 (4651916)	4651916..4652134	-	219	WP_001295676.1	CopG family transcriptional regulator	-
HZS97_RS22585 (4653738)	4653738..4654625	-	888	Protein_4416	hypothetical protein	-
HZS97_RS22590 (4655036)	4655036..4655977	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HZS97_RS22595 (4656022)	4656022..4656459	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13946.17 Da
• Isoelectric Point: 7.0126

>T167435 WP_284526834.1 NZ_CP058771:c4651456-4651082 [Shigella flexneri]
MGKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Nucleotide

• Length: 375 bp

>T167435 NZ_CP075128:831648-832061 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
GTGGTCCTGTGGCAATCTGAATTACGCGTCTCATGGCGCGCTCAGTGGATCTCGTTGCTTATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCATTGTGGATGATGCTACTGTCGCTGGTCGTGTTTGACTGCG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAAATCAAGCTGCTAATGGATGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTTTTGCGTCCGCCCTGGTTACTGAGGAGCGGGATGGTGTTGCGTTTACGCGCTGAGTCTGGGCACCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGCAGCGGAATGGCGTGAGTTGCGTCGGATAATGTTACAGCAACCAATAT
CGCGCCAACACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9244.40 Da
• Isoelectric Point: 4.2092

>AT167435 WP_001086388.1 NZ_CP058771:c4651698-4651456 [Shigella flexneri]
MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVDQYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT167435 NZ_CP075128:831401-831667 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
ATGGACATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGCGACGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTGATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A4P7TTH6