Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4338208..4338705 Replicon chromosome
Accession NZ_CP058771
Organism Shigella flexneri strain STLIN_11

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HZS97_RS21135 Protein ID WP_001173343.1
Coordinates 4338208..4338381 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HZS97_RS21140 Protein ID WP_000398619.1
Coordinates 4338409..4338705 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS97_RS21125 (4334878) 4334878..4336335 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HZS97_RS21130 (4336572) 4336572..4338089 + 1518 WP_001295074.1 lysine--tRNA ligase -
HZS97_RS21135 (4338208) 4338208..4338381 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HZS97_RS21140 (4338409) 4338409..4338705 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HZS97_RS21145 (4338932) 4338932..4339204 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
HZS97_RS21150 (4339216) 4339216..4339446 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
HZS97_RS21155 (4339627) 4339627..4341258 + 1632 WP_001216477.1 sensor histidine kinase -
HZS97_RS21160 (4341255) 4341255..4341974 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T167434 WP_001173343.1 NZ_CP058771:c4338381-4338208 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T167434 NZ_CP075128:641068-641343 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
ATGAAACAGGTATTAAAAGATTTTGACGTCGCAGACTATTTAGAGGACGAGGATGATGTTATCGCTTATCTAAATGCCGT
TATTGCCGAGGGTGACAATAAACAACTTTTCATTGCGCTGGGCAACATTGCAAGAGCAAGAAATATAAGCCGTATTTCAA
AAGAAACCGGAATCAGCAGGGAGGGAATTTATAAAGCACTTTCAGGCGATGGAAATCCATCATTTAACACCCTTTCTAAA
ATAACTAAAGCGATGGGGTTAGATCTTCACTTTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT167434 WP_000398619.1 NZ_CP058771:c4338705-4338409 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT167434 NZ_CP075128:641380-641718 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
ATGACCTATCGCGAAGATATTCGGTTGGTGGAGTATCTGATAGAGCACGAGCCGGATAGCCCGCTGATTGACATGCTCAC
AGCAAAAATTGACCGTTACGAAGATGAAGCGCCGGAGCTGGCAGAGTTTAACGCCAATATAGCCAGCACGCCGGGCGGCG
TGGCAATGTTGCGCGTACTTATGGATCAATACCATCTTGCACAAGGTAACTTTGAAAACGAGATAGGCAAGCGCTCGCTG
GTATGTCGGATACTTAACGGGCAGCGCAGGCTAACCCTTGATCATGCGCGTGCGCTGGCCAGAAGGTTTAATCTGCCTGT
AAGCGCGTTTATTGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References