Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4127401..4127977 Replicon chromosome
Accession NZ_CP058771
Organism Shigella flexneri strain STLIN_11

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HZS97_RS20105 Protein ID WP_001297643.1
Coordinates 4127690..4127977 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HZS97_RS20100 Protein ID WP_000063156.1
Coordinates 4127401..4127703 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS97_RS20080 (4122509) 4122509..4123729 + 1221 WP_284526785.1 amino acid deaminase -
HZS97_RS20085 (4123997) 4123997..4126147 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
HZS97_RS20090 (4126197) 4126197..4126400 + 204 WP_000467859.1 YbdD/YjiX family protein -
HZS97_RS20095 (4126411) 4126411..4127367 + 957 WP_001297640.1 GTPase -
HZS97_RS20100 (4127401) 4127401..4127703 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HZS97_RS20105 (4127690) 4127690..4127977 - 288 WP_001297643.1 BrnT family toxin Toxin
HZS97_RS20110 (4128174) 4128174..4129340 + 1167 WP_284526786.1 restriction endonuclease -
HZS97_RS20115 (4129385) 4129385..4129558 + 174 Protein_3939 type I restriction-modification system subunit M N-terminal domain-containing protein -
HZS97_RS20120 (4129552) 4129552..4130219 + 668 Protein_3940 DUF3387 domain-containing protein -
HZS97_RS20125 (4130434) 4130434..4130771 + 338 Protein_3941 endoribonuclease SymE -
HZS97_RS20130 (4130859) 4130859..4130906 - 48 WP_229017048.1 hypothetical protein -
HZS97_RS20135 (4131154) 4131154..4132565 + 1412 Protein_3943 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T167432 WP_001297643.1 NZ_CP058771:c4127977-4127690 [Shigella flexneri]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T167432 NZ_CP075128:640770-641066 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
ATGATTGACATAAAAACAACACCAGAGGCCGAGAAGTGGCTAAAGGGATTGAAAGACCGGGTAGCGAAATCAAAGATATT
AATCAGAATCGACAGAATGAGAACGGGGAACTTTGGTGATGTTGAGCCAGTAGGCGGCGGGTTATCTGAATTGCGTATAC
ATCACGGCAAAGGTTATCGGGTTTATTTTGGGAATAAAAACAACGAAATAATATTAATTCTTTGTGGCGGGGATAAAAGC
ACACAGCAGGATGATATACAGAAAGCGAAACAGATAGCCAAAAAATGGGGGTTTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT167432 WP_000063156.1 NZ_CP058771:c4127703-4127401 [Shigella flexneri]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT167432 NZ_CP075128:641068-641343 [Salmonella enterica subsp. diarizonae serovar 60:r:e,n,x,z15]
ATGAAACAGGTATTAAAAGATTTTGACGTCGCAGACTATTTAGAGGACGAGGATGATGTTATCGCTTATCTAAATGCCGT
TATTGCCGAGGGTGACAATAAACAACTTTTCATTGCGCTGGGCAACATTGCAAGAGCAAGAAATATAAGCCGTATTTCAA
AAGAAACCGGAATCAGCAGGGAGGGAATTTATAAAGCACTTTCAGGCGATGGAAATCCATCATTTAACACCCTTTCTAAA
ATAACTAAAGCGATGGGGTTAGATCTTCACTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References