Detailed information of TA system TA167417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1983722..1984506
• Replicon: chromosome
• Accession: NZ_CP058771
• Organism: Shigella flexneri strain STLIN_11

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: HZS97_RS09530
• Protein ID: WP_000613626.1
• Coordinates: 1983722..1984216 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: HZS97_RS09535
• Protein ID: WP_284526985.1
• Coordinates: 1984213..1984506 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS97_RS09510 (1978915)	1978915..1980012	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
HZS97_RS09515 (1980012)	1980012..1980953	+	942	WP_001295441.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
HZS97_RS09520 (1981019)	1981019..1982662	+	1644	WP_000096522.1	flagellar hook-associated protein FlgK	-
HZS97_RS09525 (1982674)	1982674..1983627	+	954	WP_001212782.1	flagellar hook-associated protein FlgL	-
HZS97_RS09530 (1983722)	1983722..1984216	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
HZS97_RS09535 (1984213)	1984213..1984506	-	294	WP_284526985.1	DUF1778 domain-containing protein	Antitoxin
HZS97_RS09540 (1984640)	1984640..1987825	-	3186	WP_000827388.1	ribonuclease E	-
HZS97_RS09545 (1988398)	1988398..1989357	+	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T167417 WP_000613626.1 NZ_CP058771:c1984216-1983722 [Shigella flexneri]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T167417 NZ_CP075125:4686549-4686860 [Salmonella enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10980.53 Da
• Isoelectric Point: 10.5423

>AT167417 WP_284526985.1 NZ_CP058771:c1984506-1984213 [Shigella flexneri]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPGEQKK

Nucleotide

• Length: 294 bp

>AT167417 NZ_CP075125:4686857-4687303 [Salmonella enterica]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: V0T0H9