Detailed information of TA system TA167416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1057629..1058212
• Replicon: chromosome
• Accession: NZ_CP058771
• Organism: Shigella flexneri strain STLIN_11

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HZS97_RS05145
• Protein ID: WP_049253269.1
• Coordinates: 1057877..1058212 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1V2T5M9
• Locus tag: HZS97_RS05140
• Protein ID: WP_000581941.1
• Coordinates: 1057629..1057877 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS97_RS05130 (1053968)	1053968..1055269	+	1302	WP_023281448.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
HZS97_RS05135 (1055317)	1055317..1057551	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
HZS97_RS05140 (1057629)	1057629..1057877	+	249	WP_000581941.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HZS97_RS05145 (1057877)	1057877..1058212	+	336	WP_049253269.1	endoribonuclease MazF	Toxin
HZS97_RS05150 (1058283)	1058283..1059074	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
HZS97_RS05155 (1059302)	1059302..1060939	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
HZS97_RS05160 (1061027)	1061027..1062325	+	1299	WP_000036723.1	phosphopyruvate hydratase	-
HZS97_RS05165 (1062385)	1062385..1063200	-	816	WP_284527011.1	YgcG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12197.18 Da
• Isoelectric Point: 8.7218

>T167416 WP_049253269.1 NZ_CP058771:1057877-1058212 [Shigella flexneri]
MVSRYVPDMGDLIWVDFDPTKGSEQARHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T167416 NZ_CP075125:c4685316-4685005 [Salmonella enterica]
ATGCAATTCATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCATCGTAGTCAACGGTATGAGGTTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9385.76 Da
• Isoelectric Point: 4.4665

>AT167416 WP_000581941.1 NZ_CP058771:1057629-1057877 [Shigella flexneri]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT167416 NZ_CP075125:c4685004-4684714 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A1V2T5M9