Detailed information of TA system TA167414

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 842596..843431
• Replicon: chromosome
• Accession: NZ_CP058771
• Organism: Shigella flexneri strain STLIN_11

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X9TM58
• Locus tag: HZS97_RS04135
• Protein ID: WP_000854761.1
• Coordinates: 842596..842973 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: HZS97_RS04140
• Protein ID: WP_001295723.1
• Coordinates: 843063..843431 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS97_RS04105 (837723)	837723..838870	-	1148	Protein_807	capsule biosynthesis protein	-
HZS97_RS04110 (838942)	838942..839925	-	984	WP_151295113.1	KpsF/GutQ family sugar-phosphate isomerase	-
HZS97_RS04115 (840706)	840706..840906	-	201	Protein_809	IS110 family transposase	-
HZS97_RS04120 (841249)	841249..841818	-	570	WP_284526922.1	DUF4942 domain-containing protein	-
HZS97_RS04125 (841903)	841903..842100	-	198	WP_000839265.1	DUF957 domain-containing protein	-
HZS97_RS04130 (842112)	842112..842599	-	488	Protein_812	DUF5983 family protein	-
HZS97_RS04135 (842596)	842596..842973	-	378	WP_000854761.1	TA system toxin CbtA family protein	Toxin
HZS97_RS04140 (843063)	843063..843431	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HZS97_RS04145 (843481)	843481..844131	-	651	Protein_815	antitoxin of toxin-antitoxin stability system	-
HZS97_RS04150 (844144)	844144..844365	-	222	WP_000692345.1	DUF987 domain-containing protein	-
HZS97_RS04155 (844434)	844434..844910	-	477	WP_001186715.1	RadC family protein	-
HZS97_RS04160 (844926)	844926..845411	-	486	WP_032153711.1	antirestriction protein	-
HZS97_RS04165 (845466)	845466..846284	-	819	WP_001234693.1	DUF932 domain-containing protein	-
HZS97_RS04170 (846375)	846375..846608	-	234	WP_284526923.1	DUF905 family protein	-
HZS97_RS04175 (846687)	846687..847143	-	457	Protein_821	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	842596..865788	23192

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14205.25 Da
• Isoelectric Point: 7.3249

>T167414 WP_000854761.1 NZ_CP058771:c842973-842596 [Shigella flexneri]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T167414 NZ_CP075125:c4252691-4252407 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT167414 WP_001295723.1 NZ_CP058771:c843431-843063 [Shigella flexneri]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT167414 NZ_CP075125:c4252923-4252681 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAGGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAGGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A1X9TM58

Antitoxin

• AlphaFold DB: S1NM52