Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 47352..48088 Replicon plasmid unnamed2
Accession NZ_CP058760
Organism Klebsiella pneumoniae strain STLIN_3

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HZT06_RS26195 Protein ID WP_003026803.1
Coordinates 47606..48088 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HZT06_RS26190 Protein ID WP_003026799.1
Coordinates 47352..47618 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT06_RS26165 (HZT06_26100) 42718..44682 + 1965 Protein_40 Tn3 family transposase -
HZT06_RS26170 (HZT06_26105) 44986..45774 + 789 WP_226322891.1 hypothetical protein -
HZT06_RS26175 (HZT06_26110) 45908..46399 + 492 WP_015065591.1 hypothetical protein -
HZT06_RS26180 (HZT06_26115) 46460..46663 + 204 WP_004213596.1 HHA domain-containing protein -
HZT06_RS26185 (HZT06_26120) 46677..46907 + 231 WP_004206660.1 hypothetical protein -
HZT06_RS26190 (HZT06_26125) 47352..47618 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HZT06_RS26195 (HZT06_26130) 47606..48088 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HZT06_RS26200 (HZT06_26135) 48247..49779 - 1533 WP_240730741.1 IS3-like element ISKpn38 family transposase -
HZT06_RS26205 (HZT06_26140) 49902..51248 + 1347 WP_240730742.1 ISNCY family transposase -
HZT06_RS26210 (HZT06_26145) 51408..52112 + 705 WP_031591821.1 toll/interleukin-1 receptor domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkF / mrkJ 1..135057 135057
- inside IScluster/Tn - - 42197..51248 9051


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T167407 WP_003026803.1 NZ_CP058760:47606-48088 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T167407 NZ_CP075124:18657-18938 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTTGTTATTTCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT167407 WP_003026799.1 NZ_CP058760:47352-47618 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT167407 NZ_CP075124:18416-18667 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References