Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5888..6413 Replicon plasmid unnamed1
Accession NZ_CP058759
Organism Klebsiella pneumoniae strain STLIN_3

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag HZT06_RS25115 Protein ID WP_013023785.1
Coordinates 5888..6193 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag HZT06_RS25120 Protein ID WP_001568025.1
Coordinates 6195..6413 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT06_RS25085 (HZT06_25025) 1559..2185 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
HZT06_RS25090 2182..2484 + 303 WP_004197636.1 hypothetical protein -
HZT06_RS25095 (HZT06_25035) 2964..3758 - 795 WP_004197635.1 site-specific integrase -
HZT06_RS25100 (HZT06_25040) 3956..4972 - 1017 WP_017899884.1 hypothetical protein -
HZT06_RS25105 (HZT06_25045) 4983..5297 - 315 WP_053389906.1 hypothetical protein -
HZT06_RS25110 (HZT06_25050) 5324..5719 - 396 WP_017899885.1 hypothetical protein -
HZT06_RS25115 (HZT06_25055) 5888..6193 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
HZT06_RS25120 (HZT06_25060) 6195..6413 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HZT06_RS25125 (HZT06_25065) 7231..7521 - 291 WP_013023783.1 hypothetical protein -
HZT06_RS25130 (HZT06_25070) 7518..8645 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
HZT06_RS25135 (HZT06_25075) 8679..10202 - 1524 WP_017899887.1 hypothetical protein -
HZT06_RS25140 (HZT06_25080) 10478..11257 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IId / floR / tet(A) / mph(A) / sul1 / qnrB91 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / blaTEM-1B / blaCTX-M-3 / qnrS1 - 1..141426 141426


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T167406 WP_013023785.1 NZ_CP058759:c6193-5888 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T167406 NZ_CP075123:c14802-14497 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT167406 WP_001568025.1 NZ_CP058759:c6413-6195 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT167406 NZ_CP075123:c15022-14804 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References