Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4457058..4457868 Replicon chromosome
Accession NZ_CP058758
Organism Klebsiella pneumoniae strain STLIN_3

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag HZT06_RS21945 Protein ID WP_002887280.1
Coordinates 4457058..4457591 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HZT06_RS21950 Protein ID WP_002887278.1
Coordinates 4457602..4457868 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT06_RS21940 (HZT06_21890) 4455889..4457010 + 1122 WP_016946712.1 cupin domain-containing protein -
HZT06_RS21945 (HZT06_21895) 4457058..4457591 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
HZT06_RS21950 (HZT06_21900) 4457602..4457868 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HZT06_RS21955 (HZT06_21905) 4457971..4459404 - 1434 WP_101999101.1 Cu(+)/Ag(+) sensor histidine kinase -
HZT06_RS21960 (HZT06_21910) 4459394..4460077 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HZT06_RS21965 (HZT06_21915) 4460250..4461635 + 1386 WP_186937423.1 efflux transporter outer membrane subunit -
HZT06_RS21970 (HZT06_21920) 4461653..4461997 + 345 WP_040148409.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T167402 WP_002887280.1 NZ_CP058758:c4457591-4457058 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T167402 NZ_CP075122:c4072579-4072295 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT167402 WP_002887278.1 NZ_CP058758:c4457868-4457602 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT167402 NZ_CP075122:c4072811-4072569 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References