Detailed information of TA system TA167385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 39799..40535
• Replicon: plasmid unnamed1
• Accession: NZ_CP058753
• Organism: Klebsiella pneumoniae strain STLIN_19

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HZT05_RS26355
• Protein ID: WP_064179701.1
• Coordinates: 40053..40535 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HZT05_RS26350
• Protein ID: WP_003026799.1
• Coordinates: 39799..40065 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT05_RS26330 (HZT05_26230)	34948..35928	+	981	Protein_36	IS5-like element ISKpn26 family transposase	-
HZT05_RS26335 (HZT05_26235)	35986..36231	+	246	WP_032238678.1	hypothetical protein	-
HZT05_RS26340 (HZT05_26240)	36200..38785	+	2586	WP_004187040.1	EAL domain-containing protein	-
HZT05_RS26345 (HZT05_26245)	38944..39648	-	705	WP_060579418.1	IS6-like element IS26 family transposase	-
HZT05_RS26350 (HZT05_26250)	39799..40065	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HZT05_RS26355 (HZT05_26255)	40053..40535	+	483	WP_064179701.1	GNAT family N-acetyltransferase	Toxin
HZT05_RS26360 (HZT05_26260)	40746..42092	+	1347	WP_240726564.1	ISNCY family transposase	-
HZT05_RS26365 (HZT05_26265)	42252..42956	+	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
HZT05_RS26370 (HZT05_26270)	43190..43300	-	111	Protein_44	IS3 family transposase	-
HZT05_RS26375 (HZT05_26280)	43575..44511	+	937	Protein_45	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..128688	128688
	-	inside	IScluster/Tn	-	-	34948..44532	9584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17307.95 Da
• Isoelectric Point: 9.5822

>T167385 WP_064179701.1 NZ_CP058753:40053-40535 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T167385 NZ_CP075120:c4072193-4071909 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT167385 WP_003026799.1 NZ_CP058753:39799-40065 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT167385 NZ_CP075120:c4072425-4072183 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66