Detailed information of TA system TA167375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 3242927..3243737
• Replicon: chromosome
• Accession: NZ_CP058752
• Organism: Klebsiella pneumoniae strain STLIN_19

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HZT05_RS16040
• Protein ID: WP_240726102.1
• Coordinates: 3242927..3243460 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: HZT05_RS16045
• Protein ID: WP_002887278.1
• Coordinates: 3243471..3243737 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT05_RS16035 (3241758)	3241758..3242879	+	1122	WP_002887282.1	cupin domain-containing protein	-
HZT05_RS16040 (3242927)	3242927..3243460	-	534	WP_240726102.1	type II toxin-antitoxin system toxin KacT	Toxin
HZT05_RS16045 (3243471)	3243471..3243737	-	267	WP_002887278.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
HZT05_RS16050 (3243840)	3243840..3245273	-	1434	WP_023288193.1	Cu(+)/Ag(+) sensor histidine kinase	-
HZT05_RS16055 (3245263)	3245263..3245946	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
HZT05_RS16060 (3246119)	3246119..3247504	+	1386	WP_004192218.1	efflux transporter outer membrane subunit	-
HZT05_RS16065 (3247522)	3247522..3247866	+	345	WP_240726103.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19837.76 Da
• Isoelectric Point: 6.2369

>T167375 WP_240726102.1 NZ_CP058752:c3243460-3242927 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T167375 NZ_CP075120:358943-359218 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT167375 WP_002887278.1 NZ_CP058752:c3243737-3243471 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT167375 NZ_CP075120:358680-358940 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1