Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 93997..94640 Replicon plasmid unnamed1
Accession NZ_CP058749
Organism Escherichia coli strain STLIN_13

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag HZS99_RS23875 Protein ID WP_001034044.1
Coordinates 93997..94413 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag HZS99_RS23880 Protein ID WP_001261286.1
Coordinates 94410..94640 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS99_RS23860 (90410) 90410..91096 + 687 Protein_102 IS1 family transposase -
HZS99_RS23865 (91350) 91350..92372 - 1023 WP_000361402.1 helicase UvrD -
HZS99_RS23870 (92357) 92357..93922 - 1566 WP_001128474.1 AAA family ATPase -
HZS99_RS23875 (93997) 93997..94413 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
HZS99_RS23880 (94410) 94410..94640 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HZS99_RS23885 (95200) 95200..95418 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
HZS99_RS23890 (95420) 95420..95725 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
HZS99_RS23895 (95726) 95726..96532 + 807 WP_000016970.1 site-specific integrase -
HZS99_RS23900 (97254) 97254..98009 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / aac(3)-IId / aph(3')-Ia / sul3 / lnu(F) / ant(3'')-Ia / tet(A) / sul2 / floR / dfrA12 / aadA2 / cmlA1 / tet(M) / sitABCD iucA / iucB / iucC / iucD / iutA 1..121453 121453
- flank IS/Tn - - 90749..91096 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T167359 WP_001034044.1 NZ_CP058749:c94413-93997 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T167359 NZ_CP075118:825270-825683 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT167359 WP_001261286.1 NZ_CP058749:c94640-94410 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT167359 NZ_CP075118:825023-825289 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References