Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4650868..4651484 Replicon chromosome
Accession NZ_CP058748
Organism Escherichia coli strain STLIN_13

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag HZS99_RS22305 Protein ID WP_001129490.1
Coordinates 4650868..4651242 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag HZS99_RS22310 Protein ID WP_001275523.1
Coordinates 4651242..4651484 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS99_RS22290 (4648371) 4648371..4649273 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
HZS99_RS22295 (4649270) 4649270..4649905 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
HZS99_RS22300 (4649902) 4649902..4650831 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
HZS99_RS22305 (4650868) 4650868..4651242 - 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
HZS99_RS22310 (4651242) 4651242..4651484 - 243 WP_001275523.1 CopG family transcriptional regulator Antitoxin
HZS99_RS22315 (4651704) 4651704..4651922 - 219 WP_001251290.1 CopG family transcriptional regulator -
HZS99_RS22320 (4652596) 4652596..4653510 - 915 WP_072649161.1 transposase -
HZS99_RS22325 (4653523) 4653523..4654410 - 888 Protein_4378 hypothetical protein -
HZS99_RS22330 (4654826) 4654826..4655767 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
HZS99_RS22335 (4655812) 4655812..4656249 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T167358 WP_001129490.1 NZ_CP058748:c4651242-4650868 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T167358 NZ_CP075118:358943-359218 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT167358 WP_001275523.1 NZ_CP058748:c4651484-4651242 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT167358 NZ_CP075118:358680-358940 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References