Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4127132..4127708 Replicon chromosome
Accession NZ_CP058748
Organism Escherichia coli strain STLIN_13

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag HZS99_RS19880 Protein ID WP_001131965.1
Coordinates 4127421..4127708 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag HZS99_RS19875 Protein ID WP_000063158.1
Coordinates 4127132..4127434 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS99_RS19855 (4122240) 4122240..4123460 + 1221 WP_000873756.1 amino acid deaminase -
HZS99_RS19860 (4123728) 4123728..4125878 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
HZS99_RS19865 (4125928) 4125928..4126131 + 204 WP_000467859.1 YbdD/YjiX family protein -
HZS99_RS19870 (4126142) 4126142..4127098 + 957 WP_001361361.1 GTPase -
HZS99_RS19875 (4127132) 4127132..4127434 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
HZS99_RS19880 (4127421) 4127421..4127708 - 288 WP_001131965.1 BrnT family toxin Toxin
HZS99_RS19885 (4127905) 4127905..4129071 + 1167 WP_000800823.1 restriction endonuclease -
HZS99_RS19890 (4129116) 4129116..4129289 + 174 Protein_3907 type I restriction-modification system subunit M N-terminal domain-containing protein -
HZS99_RS19895 (4129283) 4129283..4129951 + 669 Protein_3908 DUF3387 domain-containing protein -
HZS99_RS19900 (4130166) 4130166..4130504 + 339 WP_000132633.1 endoribonuclease SymE -
HZS99_RS19905 (4130592) 4130592..4130639 - 48 WP_229017048.1 hypothetical protein -
HZS99_RS19910 (4130887) 4130887..4132299 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T167354 WP_001131965.1 NZ_CP058748:c4127708-4127421 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T167354 NZ_CP075116:c4496560-4496249 [Salmonella enterica subsp. enterica serovar Stanleyville]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT167354 WP_000063158.1 NZ_CP058748:c4127434-4127132 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT167354 NZ_CP075116:c4496248-4495958 [Salmonella enterica subsp. enterica serovar Stanleyville]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References